MALDI-TOF MS meets WGS in a VRE outbreak investigation

被引：0

作者：

S. Schlebusch

G. R. Price

R. L. Gallagher

V. Horton-Szar

L. D. H. Elbourne

P. Griffin

D. J. Venter

S. O. Jensen

S. J. Van Hal

机构：

[1] Mater Health Services,Pathology Department

[2] The University of Queensland,School of Medicine

[3] Macquarie University,Department of Chemistry and Biomolecular Sciences

[4] Mater Health Services,Department of Medicine

[5] QIMR Berghofer,Molecular Medicine Research Group, School of Medicine

[6] MRI-UQ,Department of Microbiology and Infectious Diseases

[7] Mater Health Services,undefined

[8] Western Sydney University,undefined

[9] Antibiotic Resistance and Mobile Elements Group,undefined

[10] Ingham Institute for Applied Medical Research,undefined

[11] Royal Prince Alfred Hospital,undefined

来源：

European Journal of Clinical Microbiology & Infectious Diseases | 2017年 / 36卷

关键词：

Whole Genome Sequencing; Positive Blood Culture; Core Genome; Outbreak Investigation; Vancomycin Resistant Enterococcus;

D O I：

暂无

中图分类号：

学科分类号：

摘要：

The use of MALDI-TOF MS (matrix-assisted laser desorption/ ionization–time of flight mass spectrometry) and WGS (whole genome sequencing) has been described for identification and strain relatedness determination. We describe the complementary use of MALDI-TOF MS and WGS in a VRE (vancomycin-resistant enterococci) outbreak investigation, and discuss some of the challenges with defining strain similarity across these two platforms. Although both assays indicated multiple clusters involved in the outbreak of vancomycin resistant Enterococcus faecium isolates from positive blood cultures of four haematology–oncology patients, the small cohort and discrepancies between findings indicate the limitations of MALDI-TOF MS and the cautious interpretation of MALDI-TOF MS dendrograms during outbreaks. For definitive determination of the evolutionary distance between isolates, WGS can be used.

引用

页码：495 / 499

页数：4

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