Silicon-based microelectrode arrays for stimulation and signal recording of in vitro cultured neurons

被引:0
|
作者
HaiXian Pan
XiaoYing Lü
ZhiGong Wang
TianLing Ren
Tao Fang
Jie Zhang
ChangJian Zhou
LiGang Wang
机构
[1] Southeast University,State Key Laboratory of Bioelectronics
[2] Southeast University,Institute of RF
[3] Tsinghua University,& OE
来源
Science China Information Sciences | 2011年 / 54卷
关键词
microelectrode arrays (MEAs); extracellular recording; stimulation and recording; neurons network; in vitro;
D O I
暂无
中图分类号
学科分类号
摘要
Microelectrode arrays (MEAs) for stimulation and signal recording of in vitro cultured neurons are presented. Each MEA is composed of 60 independent electrodes with 59 working ones and one reference one. These electrodes are divided into 30 pairs. Through each pair of electrodes, four independent states can be realized to define the accessing modes of neurons cultured on the surface of the electrodes. A total MEA covers an area of 10 mm×10 mm. MEAs are fabricated in a silicon-based semiconductor process. An implemented MEA is bonded on a specially designed printed-circuit-board (PCB) and surrounded by a culture chamber. An impedance measurement has been made to verify the electrical characteristics of MEAs. The surface was modified to enhance the biocompatibility. A series of PC12 cells culture experiments validates the effectiveness of the modification. An extracellular signal recording experiment with acetylcholine (Ach) as a stimulant has been carried out, and the results show the feasibility of MEAs for extracellular action potential recording. Extracellular electrical stimulation and recording experiments have been carried out too. They indicate that MEAs can be used for extracellular stimulation, recording, simultaneous stimulation and recording, and isolation of PC12 cells network cultured in vitro.
引用
收藏
页码:2199 / 2208
页数:9
相关论文
共 50 条
  • [1] Silicon-based microelectrode arrays for stimulation and signal recording of in vitro cultured neurons
    PAN HaiXian 1
    2 Institute of RF-& OE-ICs
    3 Institute of Microelectronics
    Science China(Information Sciences), 2011, 54 (10) : 2199 - 2208
  • [2] Silicon-based microelectrode arrays for stimulation and signal recording of in vitro cultured neurons
    Pan HaiXian
    Lu XiaoYing
    Wang ZhiGong
    Ren TianLing
    Fang Tao
    Zhang Jie
    Zhou ChangJian
    Wang LiGang
    SCIENCE CHINA-INFORMATION SCIENCES, 2011, 54 (10) : 2199 - 2208
  • [3] In Vivo Validation of Custom-Designed Silicon-Based Microelectrode Arrays for Long-Term Neural Recording and Stimulation
    Han, Martin
    Manoonkitiwongsa, Panya S.
    Wang, Cindy X.
    McCreery, Douglas B.
    IEEE TRANSACTIONS ON BIOMEDICAL ENGINEERING, 2012, 59 (02) : 346 - 354
  • [4] Ruthenium oxide based microelectrode arrays for in vitro and in vivo neural recording and stimulation
    Atmaramani, Rahul
    Chakraborty, Bitan
    Rihani, Rashed T.
    Usoro, Joshua
    Hammack, Audrey
    Abbott, Justin
    Nnoromele, Patrick
    Black, Bryan J.
    Pancrazio, Joseph J.
    Cogan, Stuart F.
    ACTA BIOMATERIALIA, 2020, 101 : 565 - 574
  • [5] Galvanic modifications of multifunctional silicon-based microelectrode arrays
    Buss, G
    Ecken, H
    Winkels, S
    Schöning, MJ
    Lüth, H
    Schultze, JW
    ELECTROCHEMICAL TECHNOLOGY APPLICATIONS IN ELECTRONICS III, 2000, 99 (34): : 209 - 215
  • [6] A 64-channel ASIC for in-vitro simultaneous recording and stimulation of neurons using microelectrode arrays
    Billoint, O.
    Rostaing, J. P.
    Charvet, G.
    Yvert, B.
    2007 ANNUAL INTERNATIONAL CONFERENCE OF THE IEEE ENGINEERING IN MEDICINE AND BIOLOGY SOCIETY, VOLS 1-16, 2007, : 6070 - +
  • [7] Characterization and optimization of microelectrode arrays for in vivo nerve signal recording and stimulation
    Blau, A.
    Ziegler, Ch.
    Heyer, M.
    Endres, F.
    Schwitzgebel, G.
    Matthies, T.
    Stieglitz, T.
    Meyer, J.-U.
    Göpel, W.
    Biosensors and Bioelectronics, 1997, 12 (9-10) : 883 - 892
  • [8] Characterization and optimization of microelectrode arrays for in vivo nerve signal recording and stimulation
    Blau, A
    Ziegler, C
    Heyer, M
    Endres, F
    Schwitzgebel, G
    Matthies, T
    Stieglitz, T
    Meyer, JU
    Gopel, W
    BIOSENSORS & BIOELECTRONICS, 1997, 12 (9-10): : 883 - 892
  • [9] Characteristics of single neurons cultured on microelectrode arrays in vitro for chemical sensing
    Yang, M
    Zhang, X
    Zhang, Y
    Ozkan, CS
    IEEE SENSORS JOURNAL, 2005, 5 (04) : 690 - 695
  • [10] Gold nanostructure microelectrode arrays for in vitro recording and stimulation from neuronal networks
    Koklu, Anil
    Atmaramani, Rahul
    Hammack, Audrey
    Beskok, Ali
    Pancrazio, Joseph J.
    Gnade, Bruce E.
    Black, Bryan J.
    NANOTECHNOLOGY, 2019, 30 (23)