Optimised protocols for the metabolic profiling of S. cerevisiae by 1H-NMR and HRMAS spectroscopy

被引：0
作者：
Martina Palomino-Schätzlein
Maria Micaela Molina-Navarro
Marta Tormos-Pérez
Susana Rodríguez-Navarro
Antonio Pineda-Lucena
机构：
[1] Centro de Investigación Príncipe Felipe (CIPF),Gene Expression coupled to RNA Transport Laboratory
[2] Centro de Investigación Príncipe Felipe (CIPF),Nuclear Magnetic Resonance Service
[3] Centro de Investigación Príncipe Felipe (CIPF),Structural Biochemistry Laboratory
来源：
Analytical and Bioanalytical Chemistry | 2013年 / 405卷
关键词：
NMR; Yeast; HRMAS; Metabolomics; Stress;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
An optimised extraction protocol for the analysis of Saccharomyces cerevisiae aqueous and organic metabolites by nuclear magnetic resonance spectroscopy that allows the identification and quantification of up to 50 different compounds is presented. The method was compared with other metabolic profiling protocols for S. cerevisiae, where generally different analytical techniques are applied for metabolite quantification. In addition, the analysis of intact S. cerevisiae cells by HRMAS was implemented for the first time as a complementary method. The optimised protocols were applied to study the metabolic effect of glucose and galactose on S. cerevisiae growth. Furthermore, the metabolic reaction of S. cerevisiae to osmotic stress has been studied.
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页码：8431 / 8441
页数：10
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