Bacillus megaterium - A production system for recombinant proteins [Bacillus megaterium — ein Produktionssystem für rekombinante Proteine]

During the past years plasmid systems for recombinant protein production using Bacillus megaterium were developed. These systems are based on multicopy plasmids with functional elements for cloning in Escherichia coli and induced gene expression in B. megaterium. Different promoters, signals for protein secretion and affinity tags for purification arranged in a high variety of combinations are available. By using these systems, recombinant proteins can be produced and secreted in the grams per litre scale. © Springer-Verlag 2014.