Molecular detection of giardiasis among children at Cairo University Pediatrics Hospitals

被引：4

作者：

Ghieth M.A. ^{[1
]}

Kotb M.A. ^{[2
]}

Abu-Sarea E.Y. ^{[1
]}

El-Badry A.A. ^{[3
]}

机构：

[1] Department of Medical Parasitology, Faculty of Medicine, Beni Suef University, Beni Suef

[2] Department of Pediatrics, Kasr Al-Ainy Faculty of Medicine, Cairo University, Giza

[3] Department of Medical Parasitology, Kasr Al-Ainy Faculty of Medicine, Cairo University, Giza

来源：

Journal of Parasitic Diseases | 2016年 / 40卷 / 4期

关键词：

Copro-DNA; Giardia intestinalis; Molecular prevalence; PCR;

D O I：

10.1007/s12639-015-0714-9

中图分类号：

学科分类号：

摘要：

Giardiasis is a prevailing intestinal disease in children. This study aimed to determine molecular prevalence of Giardia intestinalis in children attending Cairo University Pediatrics Hospitals, using copro-PCR assays, conventional methods and to evaluate diagnostic effectiveness of used tests. 229 fecal samples were collected from children suffering from gastrointestinal symptoms and examined for Giardia by microscopy, Immuno-chromatographic test (ICT), copro-DNA using two PCR assays targeting tpi [nested-PCR (nPCR)] and 18S [conventional-PCR (cPCR)] genes. Out of 229 samples assessed, Giardia was diagnosed in 13.9, 17, 17.9, 4.8 % of cases using microscopy, ICT, nPCR (tpi) and cPCR (18S), respectively. Nominating both PCR assays as composite reference standard, microscopy and ICT were of reliable specificity (100 and 96.9 %) and accuracy (95.6 and 93.6 %) but of limited sensitivity (78.6 and 76.2 %). Kappa agreement showed, there was substantial agreement of ICT (0.776) and almost perfect agreement of microscopy (0.839) with PCR assays. Giardia showed a molecular prevalence of 18.3 % (42/229). ICT assay for Giardia surpassed microscopy but both couldn’t be used as a consistent single detection method due to their lowered sensitivities. nPCR targeting tpi is a reliable diagnostic test aiding to determine true prevalence of Giardia. © 2015, Indian Society for Parasitology.

引用

页码：1470 / 1474

页数：4

共 29 条

[1]

Alonzo T.A., Pepe M.S., Using a combination of reference tests to assess the accuracy of a new diagnostic test, Stat Med, 18, pp. 2987-3003, (1999)

[2]

Al-Saeed A.T., Issa S.H., Detection of Giardia lamblia antigen in stool specimens using enzyme-linked immunosorbent assay, East Med Health J, 16, 4, pp. 362-364, (2010)

[3]

Amar C.F., Dear P.H., Pedaza-Diaz S., Looker N., Linnane E., McLauchlin J., Sensitive PCR-RFLP assay for detection and genotyping of Giardia duodenalis in human feces, Clin Microbiol J, 40, 2, pp. 446-452, (2002)

[4]

Amjad I.A., Hussein A.B., Yamaguchi T., Nakamoto K., Motohiro Iseki M., Tokoro M., Multiple-subgenotype infections of Giardia intestinalis detected in Palestinian clinical cases using a subcloning approach, Parasitol Internat, 58, pp. 258-262, (2009)

[5]

Bertrand I., Albertini L., Schwartzbord A., Comparison of two target genes for detection and genotyping of Giardia lamblia in human feces by PCR and PCR-restriction fragment length polymorphism, J Clin Microbiol, 43, 12, pp. 5940-5944, (2005)

[6]

Elsafi S.H., Al-Maqati T.N., Hussein M.I., Adam A.A., Hassan M.M., Al Zahrani E.M., Comparison of microscopy, rapid immunoassay, and molecular techniques for the detection of Giardia lamblia and Cryptosporidium parvum, Parasitol Res, 112, 4, pp. 1641-1646, (2013)

[7]

Farthing M.J., Mata L., Urrutia J.J., Kronmal R.A., Natural history of Giardia infection of infants and children in rural Guatemala and its impact on physical growth, Am J Clin Nutr, 43, 3, pp. 395-405, (1986)

[8]

Fawzi M., El-Sahn A.A., Ibrahim H.F., Shehata A.I., Vegetable transmitted parasites among inhabitants of El-Prince, Alexandria and its relation to housewives’ knowledge and practices, J Egypt Public Health Assoc, 79, pp. 13-29, (2004)

[9]

Foronda P.M., Argues N., Abreu-Acosta M.V., Periago M.A., Valero B.V., Mas-coma S., Identification of genotypes of Giardia intestinalis of human isolates in Egypt, Parasitol Res, 103, pp. 1177-1181, (2008)

[10]

Gelanew T., Lalle M., Hailu A., Pozio E., Caccio S.M., Molecular characterization of human isolates of Giardia duodenalis from Ethiopia, Acta Trop, 102, pp. 92-99, (2007)

← 1 2 3 →