Kinetics and Mechanisms of Oxidative Hemolysis of Erythrocytes under the Action of Azo- and Peroxide Initiator

被引：0

作者：

Sokolova E.M. ^{[1
]}

Dubenskaia N.A. ^{[2
]}

Psikha B.L. ^{[1
]}

Neshev N.I. ^{[1
]}

机构：

[1] Federal Research Center of Problems of Chemical Physics and Medicinal Chemistry, Russian Academy of Sciences, 1 prosp. Akademika Semenova, Moscow oblast, Chernogolovka

[2] Faculty of Fundamental Physical and Chemical Engineering, Lomonosov Moscow State University, Moscow

来源：

Biophysics | 2023年 / 68卷 / 4期

关键词：

erythrocytes; hemoglobin; lipid peroxidation; oxidative hemolysis;

D O I：

10.1134/S0006350923040206

中图分类号：

学科分类号：

摘要：

Abstract: The kinetics of oxidative hemolysis of the 0.2% mouse erythrocytes suspension has been studied over a wide range of concentrations under the action of radical-forming initiators 2,2'-azobis(2-amidinopropan) dihydrochloride and tert-butyl hydroperoxide. Hemolysis of erythrocytes proceeded upon activation lipid peroxidation in erythrocyte membranes. In the case of tert-butyl hydroperoxide, oxidative processes also developed in the soluble part of the cell, leading to the formation of insoluble hemoglobin aggregates. Period of hemolysis induction (time of reaching 10% hemolysis) under the influence of 2,2'-azobis(2-amidinopropane) dihydrochloride decreases inversely to the square root of the initiator concentration, which is consistent with the classical theory of radical chain oxidation of hydrocarbons. In the case of tert-butyl hydroperoxide this pattern is not observed. In the erythrocyte model of testing natural and synthetic compounds for antioxidant activity it is preferable to use 2,2'-azobis (2-amidinopropane) dihydrochloride as the initiator of lipid peroxidation. © 2023, Pleiades Publishing, Inc.

引用

页码：570 / 575

页数：5

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