ADAM33 haplotypes are associated with asthma in a large Australian population

被引:0
作者
Mary-Anne Kedda
David L Duffy
Bernadette Bradley
Robyn E O’Hehir
Philip J Thompson
机构
[1] The Co-operative Research Centre (CRC) for Asthma,Department of Allergy
[2] The Asthma and Allergy Research Institute (Inc.),undefined
[3] Sir Charles Gairdner Hospital,undefined
[4] The Centre for Asthma,undefined
[5] Allergy and Respiratory Research,undefined
[6] University of Western Australia,undefined
[7] The Western Australian Institute for Medical Research and Centre for Medical Research,undefined
[8] School of Public Health,undefined
[9] Queensland University of Technology,undefined
[10] School of Life Sciences and Institute of Health and Biomedical Innovation,undefined
[11] Queensland University of Technology,undefined
[12] Genetic Epidemiology Laboratory,undefined
[13] Queensland Institute of Medical Research,undefined
[14] Immunology and Respiratory Medicine,undefined
[15] The Alfred Hospital and Monash University,undefined
来源
European Journal of Human Genetics | 2006年 / 14卷
关键词
polymorphism; MALDI-TOF; asthma; ADAM33; haplotype;
D O I
暂无
中图分类号
学科分类号
摘要
The ADAM33 gene has recently been identified as being a potentially important asthma candidate gene, and polymorphisms in this gene have been shown to be associated with asthma and bronchial hyperresponsiveness in Caucasian individuals from several populations. We performed chip-based matrix-assisted laser desorption/ionisation time-of-flight (MALDI-TOF) mass spectrometry using the MassARRAY system and multiplexed genotyping assays to investigate the association between 10 single nucleotide polymorphisms (SNPs) in the ADAM33 gene (F_+1, Q_−1, S_1, ST_+4, ST_+7, V_−2, V_−1, V_2, V_4, V_5) and asthma and asthma severity in a large Australian Caucasian population of nonasthmatic controls (n=473), and patients with mild (n=292), moderate (n=238) and severe (n=82) asthma. No significant association was found between any one of the 10 SNPs and asthma or asthma severity, however, there was a significant global haplotypic association with asthma (P=0.0002) and disease severity (P=0.0001), driven by the combination of two key SNPs, V_−1 and ST_+7. A meta-analysis of all the genetic studies conducted to date found significant between-study heterogeneity, likely to reflect population stratification. Our analysis of ADAM33 haplotypes further suggests a likely role for ADAM33 in the asthma phenotype, although it does not exclude an association with another locus in linkage disequilibrium with ADAM33.
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页码:1027 / 1036
页数:9
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