Deposition of callose in treatment of cells of the tomato plant (Lycopersicon esculentum L.) with biotic elicitors

被引:0
|
作者
V. I. Emel’yanov
Zh. N. Kravchuk
S. A. Polyakovskii
A. P. Dmitriev
机构
[1] National Academy of Sciences of Ukraine,Institute of Cellular Biology and Genetic Engineering
来源
Cytology and Genetics | 2008年 / 42卷
关键词
Chitosan; Suspension Culture; Glucan; Tomato Plant; Chitinase Activity;
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学科分类号
摘要
The dynamics of induced accumulation of callose in tomato cells is studied. Localization of callose in the cells is studied by methods of fluorescence microscopy and the time needed for quantitative determination of callosa is optimized. The quantity of callose in tomato cells treated with different biotic elicitors is established. A nonlinear dependence of the deposition of callose on the concentration of chitin oligomers containing 3–5 fragments of N-acetylglucosamine is established and a proportional increase in the content of callose in the cells with increasing concentration of chitin dimer and chitosan in the culture medium is demonstrated.
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页码:90 / 95
页数:5
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