Expression of a fungal laccase fused with a bacterial cellulose-binding module improves the enzymatic saccharification efficiency of lignocellulose biomass in transgenic Arabidopsis thaliana

被引:0
作者
Ryota Iiyoshi
Taichi Oguchi
Toru Furukawa
Yosuke Iimura
Yukihiro Ito
Tomonori Sonoki
机构
[1] Hirosaki University,Graduate School of Agriculture and Life Science
[2] University of Tsukuba,Tsukuba Plant Innovation Research Center (T
[3] National Institute of Advanced Industrial Science and Technology,PIRC), Faculty of Life and Environmental Sciences
[4] Tohoku University,Graduate School of Agricultural Science
来源
Transgenic Research | 2017年 / 26卷
关键词
Laccase; Cellulose-binding module; Saccharification; Cell wall composition; Lignocellulosic biomass;
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中图分类号
学科分类号
摘要
Delignification is effective for improving the saccharification efficiency of lignocellulosic biomass materials. We previously identified that the expression of a fungal laccase (Lac) fused with a bacterial cellulose-binding module domain (CBD) improved the enzymatic saccharification efficiency of rice plants. In this work, to evaluate the ability of the Lac-CBD fused chimeric enzyme to improve saccharification efficiency in a dicot plant, we introduced the chimeric gene into a dicot model plant, Arabidopsis thaliana. Transgenic plants expressing the Lac-CBD chimeric gene showed normal morphology and growth, and showed a significant increase of enzymatic saccharification efficiency compared to control plants. The transgenic plants with the largest improvement of enzymatic saccharification efficiency also showed an increase of crystalline cellulose in their cell wall fractions. These results indicated that expression of the Lac-CBD chimeric protein in dicotyledonous plants improved the enzymatic saccharification of plant biomass by increasing the crystallinity of cellulose in the cell wall.
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页码:753 / 761
页数:8
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