Determination of partial genomic sequences and development of a CAPS system of the S-RNase gene for the identification of 22 S haplotypes of apple (Malus × domestica Borkh.)

被引：0
作者：
Hoytaek Kim
Hiroyuki Kakui
Nobuhiro Kotoda
Yutaka Hirata
Takato Koba
Hidenori Sassa
机构：
[1] Chiba University,Faculty of Horticulture
[2] National Institute of Fruit Tree Science,Fruit Genome Research Team
[3] Chiba University,Graduate School of Science and Technology
[4] National Institute of Fruit Tree Science,Department of Apple Research
[5] National Agricultural Research Organization,Fruit Genome Research Team
[6] National Institute of Fruit Tree Science,Graduate School of Agriculture
[7] Tokyo University of Agriculture and Technology,undefined
来源：
Molecular Breeding | 2009年 / 23卷
关键词：
Apple; CAPS; genotype; Self-incompatibility; S-RNase;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Information about self-incompatibility (S) genotypes of apple cultivars is important for the selection of pollen donors for fruit production and breeding. Although S genotyping systems using S haplotype-specific PCR of S-RNase, the pistil S gene, are useful, they are sometimes associated with false-positive/negative problems and are unable to identify new S haplotypes. The CAPS (cleaved amplified polymorphic sequences) system is expected to overcome these problems, however, the genomic sequences needed to establish this system are not available for many S-RNases. Here, we determined partial genomic sequences of eight S-RNases, and used the information to design new primer and to select 17 restriction enzymes for the discrimination of 22 S-RNases by CAPS. Using the system, the S genotypes of three cultivars were determined. The genomic sequence-based CAPS system would be useful for S genotyping and analyzing new S haplotypes of apple.
引用
收藏
相关论文
共 132 条
  • [1] Bošković R(1999)Correlation of stylar ribonuclease isoenzymes with incompatibility alleles in apple Euphytica 107 29-43
  • [2] Tobutt KR(2003)New findings in apple S-genotype analysis resolve previous confusion and request the re-numbering of some S-alleles Theor Appl Genet 106 703-714
  • [3] Broothaerts W(2007)Identification of S-alleles in 40 apple ( Acta Hortic 760 111-115
  • [4] Ershadi A(1995) ×  Theor Appl Genet 91 691-698
  • [5] Talaii AR(2006) Borkh) cultivars by allele-specific PCR amplification J Plant Biol 49 448-454
  • [6] Janssens GA(2007)A molecular method for Breed Sci 57 159-164
  • [7] Goderis IJ(2008)-allele identification in apple based on allele-specific PCR J Plant Biol 51 202-208
  • [8] Broekaert WF(2000)Determination of self-incompatibility genotypes of Korean apple cultivars based on S-RNase PCR HortScience 35 712-715
  • [9] Broothaerts W(1939)Cloning of a new Landw Jb Schweiz 53 160-191
  • [10] Kim HT(1999) and development of a PCR-RFLP system for determination of the J Jpn Soc Hortic Sci 68 94-86
12345678910