Spontaneous spectinomycin resistance mutations detected after biolistic transformation of Daucus carota L.

被引：1

作者：

Filipenko E.A. ^{[1
]}

Sidorchuk Y.V. ^{[1
]}

Titov I.I. ^{[1
]}

Maltsev V.P. ^{[2
]}

Deineko E.V. ^{[1
]}

机构：

[1] Institute of Cytology and Genetics, Siberian Branch, Russian Academy of Sciences, Novosibirsk 630090

[2] Institute of Chemical Kinetics and Combustion, Siberian Branch, Russian Academy of Sciences, Novosibirsk 630090

来源：

Physiology and Molecular Biology of Plants | 2011年 / 17卷 / 1期

关键词：

Callus; Daucus carota L; rrn16; gene; Spectinomycin resistance; Spontaneous mutation;

D O I：

10.1007/s12298-011-0051-0

中图分类号：

学科分类号：

摘要：

Spectinomycin resistant mutant carrot (Daucus carota L.) callus lines detected in the experiments on biolistic transformation of plastome were analyzed. It has been found that this antibiotic resistance is determined by point nucleotide substitutions at two distinct sites of the chloroplast gene rrn16, coding for 16S rRNA, namely, G1012T, G1012C, and A1138G. The detected mutations are localized to the 16S rRNA region forming helix h34, which contains spectinomycin binding site, and lead to its destabilization by several kilocalories per mole. Comparative analysis of rrn16 gene sequences has demonstrated conservation of the positions of the nucleotide substitutions determining this antibiotic resistance in carrot (D. carota L.), tobacco (Nicotiana tabacum L.), and bladder pod (Lesquerella fendleri L.), as well as in Escherichia coli. © 2011 Prof. H.S. Srivastava Foundation for Science and Society.

引用

页码：79 / 86

页数：7

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