Fast supercritical fluid chromatography of polymers using packed capillary columns

被引：0
|
作者：
Wu, N. [3 ]
Lee, R. [2 ]
Lee, M.L. [1 ]
机构：
[1] Department of Chemistry and Biochemistry, Brigham Young University, Provo, UT 84602-5700, United States
[2] ALZA Corporation, Mountain View, CA 94043-1218, United States
[3] Merck Research Laboratories, Rahway, NJ 07065, United States
来源：
Chromatographia | 2000年 / 53卷 / 3-4期
关键词：
Supercritical fluid chromatography; Packed capillary; Perfluorinated polyethers; Polymethylsiloxane; Carbon dioxide; Fast separations;
D O I：
10.1007/bf02491570
中图分类号：
学科分类号：
摘要：
Fast separations of perfluorinated polyethers and polymethylsiloxanes that are composed of 50–80 oligomers were demonstrated in packed capillary column supercritical fluid chromatography (SFC) using a carbon dioxide mobile phase. Separations were accomplished within 10 min using a 13 cm×250 μm i.d. column packed with 2 μm porous octadecyl bonded silica (ODS) particles. Effects of particle diameter of the packing material and pressure programming on separation were investigated, and packed column SFC was compared with open tubular column SFC. Results show that as the particle diameter was decreased from 5 to 3 to 2 μm and the column length was reduced from 85 to 43 to 13 cm, the separation time could be reduced from 70 to 20 to 10 min while still maintaining similar separation (resolution). Short columns packed with small porous particles are very suitable for fast SFC separations of polymers.
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页码：197 / 200
页数：3
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