In vitro regeneration of peanut (Arachis hypogaea L.) through organogenesis: Effect of culture temperature and silver nitrate

被引：22
作者：
Pestana M.C. [1 ,3 ]
Lacorte C. [2 ]
De Freitas V.G. [1 ]
De Oliveira D.E. [1 ]
Mansur E. [3 ]
机构：
[1] Univ. Federal do Rio de Janeiro, Instituto de Biologia, LGMV/DG, Rio de Janeiro
[2] CNPH/EMBRAPA, 70.359-970 Brasília, DF
[3] Univ. do Estado do Rio de Janeiro, Inst. de Biol. - LABMIT - R. Sao F., Xavier 524. PHLC, 20550.013 Rio de Janeiro, RJ
来源：
In Vitro Cellular & Developmental Biology - Plant | 1999年 / 35卷 / 3期
关键词：
Organogenesis; Peanut; Silver nitrate; Temperature;
D O I：
10.1007/s11627-999-0080-3
中图分类号：
学科分类号：
摘要：
The effect of culture temperature on the morphogenetic response of Arachis hypogaea was studied. Cotyledons were cultivated on MS medium supplemented with 110 μM 6-benzyladenine. Leaf explants were cultivated in the presence of the same growth regulator at 22 μM. Cultures were incubated at temperatures of 25, 28, and 35 ± 5°C. Both direct organogenesis from cotyledons and development of organogenic calluses from leaves showed optimal rates at 35 ± 5°C. The highest frequency of elongation of buds into shoots from leaf-derived calluses occurred in the presence of 5 μM AgNO3. At the best culture temperature, an average of 95% of shoots formed roots on growth-regulator-free MS medium. Plants were successfully transferred to soil, showing normal phenotypes.
引用
收藏
页码：214 / 216
页数：2
相关论文
共 18 条
  • [1] Baker C.M., Wetzstein H.Y., Influence of auxin type and concentration on peanut somatic embryogenesis, Plant Cell Tissue Organ Cult., 36, pp. 361-368, (1994)
  • [2] Baker C.M., Durham R.E., Burns J.M., Parrot W.A., Wetzstein H.Y., High frequency somatic embryogenesis in peanut (Arachis hypogaea L.) using mature, dry seed, Plant Cell Rep., 15, pp. 38-42, (1995)
  • [3] Cheng M., Hsi D.C.H., Phillips G.C., In vitro regeneration of Valencia-type peanut (Arachis hypogaea L.) from cultured petiolules, epicotyl sections and other seedling explants, Peanut Sci., 19, pp. 82-87, (1992)
  • [4] Chengalrayan K., Mhaske V.B., Harza S., In vitro regulation of morphogenesis in peanut (Arachis hypogaea L.), Plant Sci., 110, pp. 259-268, (1995)
  • [5] Chraibi K.M.B., Latche A., Roustan J.-P., Fallot J., Stimulation of shoot regeneration from cotyledons of Helianthus annuus by the ethylene inhibitors, silver and cobalt, Plant Cell Rep., 10, pp. 204-207, (1991)
  • [6] Decout E., Dubois T., Guedira M., Dubois J., Audran J.C., Vasseur J., Role of temperature as a triggering signal for organogenesis or somatic embryogenesis in wounded leaves of chicory cultured in vitro, J. Exp. Bot., 45, pp. 1859-1865, (1994)
  • [7] Dunbar K.B., Pittman R.N., Adventitious shoot formation from mature leaf explants of Arachis species, Crop Sci., 32, pp. 1353-1356, (1992)
  • [8] Duran-Vila N., Gogorcena Y., Ortega V., Ortiz J., Navarro L., Morphogenesis and tissue culture of sweet orange (Citrus sinensis (L.) osb.): Effect of temperature and photosynthetic radiation, Plant Cell Tissue Organ Cult., 29, pp. 11-18, (1992)
  • [9] Eapen S., George L., Plant regeneration from leaf dises of peanut and pigeonpea: Influence of benzyladenine, indoleacetic acid and indoleacetic acid-amino acid conjugates, Plant Cell Tissue Organ Cult., 35, pp. 223-227, (1993)
  • [10] Freitas V.G., Lacorte C., Sachetto-Martins G., Krul W.R., Oliveira D.E., Neves L.J., Mansur E., Identification of competent cells for Agrobacterium-transformation an in vitro regeneration in peanut leaf and cotyledon explants, Braz. J. Plant. Physiol., 9, pp. 157-167, (1997)
12