Preparation, characterization, and application of a new stationary phase containing different kinds of amine groups

被引：0
作者：
Luan Xu
Rui Peng
Xingmei Guan
Wanjin Tang
Xiaoyan Liu
Haixia Zhang
机构：
[1] Lanzhou University,Key Laboratory of Nonferrous Metal Chemistry and Resources Utilization of Gansu Province
来源：
Analytical and Bioanalytical Chemistry | 2013年 / 405卷
关键词：
Stationary phase; Hydrophilic interaction chromatography; Mixed-mode retention mechanism; Tertiary amine group; Primary amine group;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
A new stationary phase containing primary amine and tertiary amine groups was synthesized and its structure was confirmed by infrared spectra, elemental analysis, and zeta potential. This new material was packed into a stainless steel column and the effect of acetonitrile (ACN) content in mobile phase on retention was evaluated using probes of nucleosides, sulfanilamides, and quinolone compounds. This showed hydrophilic interaction, ion interaction, or a mixed-mode retention mechanism for different kinds of solutes, respectively. The effects of the pH and ion strength of the mobile phase were investigated to further understand the retention mechanism of the new stationary phase. The interaction forces caused by the new phase included adsorption, electrostatic function, and ion exchange. The new material could be used as a stationary phase in different high-performance liquid chromatography modes.
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页码：8311 / 8318
页数：7
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