Effects of Chromium Picolinate on Oxidative Damage in Primary Piglet Hepatocytes

被引:4
作者
Gao-Yi Tan
Jin-Ming Bi
Min-Hong Zhang
Jing-Hai Feng
Peng Xie
Shan-Shan Zheng
机构
[1] Chinese Academy of Agricultural Sciences,State Key Laboratory of Animal Nutrition, Institute of Animal Sciences
来源
Biological Trace Element Research | 2008年 / 126卷
关键词
Chromium picolinate; Piglet hepatocyte; Reactive oxygen species; Oxidative damage;
D O I
暂无
中图分类号
学科分类号
摘要
Chromium picolinate is a popular nutritional supplement whose safety has been questioned because of the potential risk of oxidative DNA damage. To investigate this possibility, a dose-dependent study was performed in piglet hepatocyte cultures in which low (8 μM), medium (200 μM), and high (400 μM) doses of chromium picolinate were tested and compared to untreated controls. After 48 h incubation, there were no significant differences in the levels of intracellular reactive oxygen species, medium lactate dehydrogenase activity, and comet indicators between the three experimental groups and controls (p > 0.05). In the 8 μM-treated group, the intracellular malondialdehyde content was significantly decreased relative to controls (p < 0.05). All of the studied parameters showed a dose-dependent increase that was statistically significant between the low and high doses (p < 0.05). These results suggest that: (1) chromium picolinate may affect the oxidative status of piglet hepatocytes; (2) the appropriate dose (approximately physiological concentration) of chromium picolinate can inhibit lipid peroxidation, and (3) high doses of chromium picolinate have no significant effects on oxidative damage in piglet hepatocytes, but the existing evidence also imply that exposure to a higher dose appears to be unwarranted.
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页码:69 / 79
页数:10
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