Functional characterization of human Kindlin-2 core promoter identifies a key role of SP1 in Kindlin-2 transcriptional regulation

被引：0
作者：
Ammad Aslam Khan
Takashi Shimokawa
Staffan Strömblad
Hongquan Zhang
机构：
[1] Karolinska Institutet,Center for Biosciences, Department of Biosciences and Nutrition
[2] The Ministry of Education,Peking University School of Basic Medical Sciences, Laboratory of Molecular Cell Biology and Tumor Biology and Key Laboratory of Carcinogenesis and Translational Research
来源：
Cellular & Molecular Biology Letters | 2011年 / 16卷
关键词：
Kindlin-2; FERMT2; PLEKHC1; Mig-2; Transcription factor SP1; Promoter; Transcription start site; Gene regulation; -acting elements; CpG island; Cell migration; Integrin; Gene expression;
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摘要：
Kindlin-2 is a recently identified FERM and PH domain containing integrin interacting protein. Kindlin-2 is ubiquitously expressed in normal tissues. So far, much effort has been spent exploring the functional aspects of Kindlin-2. However, the transcriptional regulation of Kindlin-2 has not yet been investigated. In this study we identified and functionally characterized the promoter of the human Kindlin-2 gene. We show that the core promoter of Kindlin-2 is a 39 base pair long GC rich fragment located −122/-83 upstream of the Kindlin-2 transcription start site. Functional characterization of this core promoter region by both in silico as well as in vitro/in vivo analysis shows that the transcription factor SP1 plays an important role in regulation of Kindlin-2 expression.
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