Vascular endothelial growth factor expression in rat skin incision wound

被引：0
作者：
Makoto Nogami
Tomoaki Hoshi
Mayumi Kinoshita
Tomomi Arai
Michiho Takama
Ichiro Takahashi
机构：
[1] Teikyo University School of Medicine,Department of Legal Medicine
[2] Teikyo University School of Medicine,Central Laboratory for Electron Microscopy
来源：
Medical Molecular Morphology | 2007年 / 40卷
关键词：
Vascular endothelial growth factor; Skin; Wound; Immunohistochemistry; mRNA;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Vascular endothelial growth factor (VEGF) is a glycoprotein that enhances vascular permeability, induces chemotaxis and activation of monocytes/macrophages, and promotes growth of vascular endothelial cells. We report that infiltrating polymorphonuclear leukocytes in an incision wound in rat skin express VEGF from 1 day after the injury, as shown by immunohistochemistry. VEGF is also present in macrophages, fibroblast-like cells, and endothelial cells 3 and 7 days after the injury. mRNA for VEGF is statistically significantly increased in the wound area in the tissue 1 day after the skin incision compared with 3 and 7 days after the incision. The VEGF protein content in the wound tissue is statistically significantly higher in the wound than in control skin at 1 and 3 days after skin incision. Our results indicate that VEGF is produced by inflammatory cells to induce vascularization in the early stage of the wound healing process.
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页码：82 / 87
页数：5
相关论文
共 88 条
[1]  
Martin P(1997)Wound healing: aiming for perfect skin regeneration Science 276 75-81
[2]  
Iijima K(1996)Activation-induced expression of vascular permeability factor by human peripheral T cells: a non-radioisotopic semiquantitative reverse transcription-polymerase chain reaction assay J. Immunol Methods 196 199-209
[3]  
Yoshikawa N(2005)Angiogenesis of glioma: evaluation of ultrastructural characteristics of microvessels and tubular bodies (Weibel–Palade) in endothelial cells and immunohistochemical findings with VEGF and p53 protein Med Mol Morphol 38 36-42
[4]  
Nakamura H(2004)Forensic application of VEGF expression to skin wound age determination Int J Legal Med 118 320-325
[5]  
Miyagami M(2002)Retroviral delivery of dominant-negative vascular endothelial growth factor receptor type 2 to murine wounds inhibits wound angiogenesis Wound Repair Regen 10 222-229
[6]  
Katayama Y(2006)Comparison of relative mRNA quantification models and the impact of RNA integrity in quantitative real-time RT-PCR Biotechnol Lett 28 1601-1613
[7]  
Hayashi T(2002)Immunohistochemical study of basic fibroblast growth factor and vascular endothelial growth factor expression for age determination of cutaneous wounds Am J Forensic Med Pathol 23 264-267
[8]  
Ishida Y(2006)Neutrophils and macrophages promote angiogenesis in the early stage of endometriosis in a mouse model Endcrinology 147 1278-1286
[9]  
Kimura A(2004)CXCL1/Macrophage inflammatory protein-2-induced angiogenesis in vivo is mediated by neutrophil-derived vascular endothelial growth factor-A J Immunol 172 5032-5040
[10]  
Takayasu T(2006)Effects of peritoneal fluid from endometriosis patients on the release of vascular endometrial growth factor by neutrophils and monocytes Hum Reprod 21 1846-1855
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