Cell size as a tool to predict the production of recombinant protein by the insect-cell baculovirus expression system

被引：0
作者：
Laura A. Palomares
Juan Carlos Pedroza
Octavio T. Ramírez
机构：
[1] Universidad Nacional Autónoma de México,Departamento de Bioingeniería, Instituto de Biotecnología
[2] Universidad Autónoma del Estado de Morelos,Centro de Investigación en Biotecnología
来源：
Biotechnology Letters | 2001年 / 23卷
关键词：
baculovirus; culture monitoring; insect cell size; metabolism; recombinant protein;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
The increase of Sf9 cell diameter after infection with a recombinant baculovirus encoding VP8 protein of rotavirus can be used to predict culture productivity. A direct proportional correlation between the increase in cell size and VP8 concentration was obtained when manipulating selected medium components. Only yeast extract increased (38%) VP8 concentration, while fetal bovine serum increased (55%) the maximum cell concentration. An inexpensive and simplified culture media can thus be designed without detriment to protein yields.
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页码：359 / 364
页数：5
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