Cell size as a tool to predict the production of recombinant protein by the insect-cell baculovirus expression system

被引：0
作者：
Laura A. Palomares
Juan Carlos Pedroza
Octavio T. Ramírez
机构：
[1] Universidad Nacional Autónoma de México,Departamento de Bioingeniería, Instituto de Biotecnología
[2] Universidad Autónoma del Estado de Morelos,Centro de Investigación en Biotecnología
来源：
Biotechnology Letters | 2001年 / 23卷
关键词：
baculovirus; culture monitoring; insect cell size; metabolism; recombinant protein;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
The increase of Sf9 cell diameter after infection with a recombinant baculovirus encoding VP8 protein of rotavirus can be used to predict culture productivity. A direct proportional correlation between the increase in cell size and VP8 concentration was obtained when manipulating selected medium components. Only yeast extract increased (38%) VP8 concentration, while fetal bovine serum increased (55%) the maximum cell concentration. An inexpensive and simplified culture media can thus be designed without detriment to protein yields.
引用
收藏
页码：359 / 364
页数：5
相关论文
共 48 条
[1]  
Chai H(1996)Insect cell line dependent gene expression of recombinant tumor necrosis factor- Enzyme Microbiol. Technol. 18 126-132
[2]  
Al-Rubeai M(1995)The growth and nutrient utilization of insect cell line J. Biotechnol. 40 187-198
[3]  
Chua KL(1993) Sf9 in batch and continuous culture Biotechnol. Bioeng. 42 697-707
[4]  
Oh SKW(1991)Utilization of glucose and amino acids in insect cell cultures: quantifying the metabolic flows within the primary pathways and medium development Vitro Cell Dev. Biol. 24A 470-478
[5]  
Yap MGS(1970)Replacement of vertebrate serum with lipids and other factors in the culture of invertebrate cells, tissue, parasites and pathogens Trichoplusia ni. Nature 226 466-467
[6]  
Drews M(1991)Established insect cell line from the cabbage looper Cytotechnology 5 15-30
[7]  
Paalme T(1996)Nutrient optimization for high density biological production applications Biotechnol. Bioeng. 50 36-48
[8]  
Vilu R(1999)Online monitoring of respiration in recombinant-baculovirus infected and uninfected insect cell bioreactor cultures J. Biotechnol. 72 61-75
[9]  
Ferrance J(1996)An insight into insect cell metabolism through selective nutrient manipulation Biotechnol. Lett. 18 765-770
[10]  
Goel A(1995)Glutamine is not an essential amino acid for Sf-9 insect cells Appl. Microbiol. Biotechnol. 43 1-8
12345