Multifunctional carbon-nanotube cellular endoscopes

被引：0

作者：

Singhal R. ^{[1
,5
]}

Orynbayeva Z. ^{[1
,2
,5
]}

Sundaram R.V.K. ^{[3
,5
]}

Niu J.J. ^{[1
,5
]}

Bhattacharyya S. ^{[1
,5
,6
]}

Vitol E.A. ^{[4
,5
]}

Schrlau M.G. ^{[1
,5
]}

Papazoglou E.S. ^{[3
,5
]}

Friedman G. ^{[4
,5
]}

Gogotsi Y. ^{[1
,5
]}

机构：

[1] Department of Materials Science and Engineering, Drexel University, Philadelphia

[2] Department of Biochemistry and Molecular Biology, Drexel University, Philadelphia

[3] School of Biomedical Engineering, Science and Health Systems, Drexel University, Philadelphia

[4] Department of Electrical and Computer Engineering, Drexel University, Philadelphia

[5] A.J. Drexel Nanotechnology Institute, Drexel University, Philadelphia

[6] Department of Chemical Sciences, Indian Institute of Science Education and Research, Kolkata, Mohanpur - 741252, Nadia, W.B.

来源：

Nature Nanotechnology | 2011年 / 6卷 / 1期

基金：

美国国家科学基金会;

关键词：

D O I：

10.1038/nnano.2010.241

中图分类号：

学科分类号：

摘要：

Glass micropipettes, atomic force microscope tips and nanoneedles can be used to interrogate cells, but these devices either have conical geometries that can damage cells during penetration or are incapable of continuous fluid handling. Here, we report a carbon-nanotube-based endoscope for interrogating cells, transporting fluids and performing optical and electrochemical diagnostics at the single organelle level. The endoscope, which is made by placing a multiwalled carbon nanotube (length, 50-60 μm) at the tip of a glass pipette, can probe the intracellular environment with a spatial resolution of ∼100 nm and can also access organelles without disrupting the cell. When the nanotube is filled with magnetic nanoparticles, the endoscope can be remotely manoeuvered to transport nanoparticles and attolitre volumes of fluids to and from precise locations. Because they are mounted on conventional glass micropipettes, the endoscopes readily fit standard instruments, creating a broad range of opportunities for minimally invasive intracellular probing, drug delivery and single-cell surgery. © 2011 Macmillan Publishers Limited. All rights reserved.

引用

页码：57 / 64

页数：7

共 33 条

[31]

Hayakawa K., Tatsumi H., Sokabe M., Actin stress fibers transmit and focus force to activate mechanosensitive channels, J. Cell Sci., 121, pp. 496-503, (2008)

[32]

Laffafian I., Hallett M.B., Gentle microinjection for myeloid cells using SLAM, Blood, 95, pp. 3270-3271, (2000)

[33]

Pozzan T., Rizzuto R., Volpe P., Meldolesi J., 1Molecular and cellular physiology of intracellular calcium stores, Physiol. Rev., 74, pp. 595-636, (1994)

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