Characterization of a putative type IV aminophospholipid transporter P-type ATPase

被引：0

作者：

Stéphane Flamant

Pascale Pescher

Brigitte Lemercier

Mathieu Clément-Ziza

François Képès

Marc Fellous

Geneviève Milon

Gilles Marchal

Claude Besmond

机构：

[1] Hôpital Necker-Enfants Malades,

[2] INSERM U393,undefined

[3] Tour Lavoisier,undefined

[4] 2 ème étage,undefined

[5] 149 rue de Sèvres,undefined

[6] 75015 Paris,undefined

[7] France,undefined

[8] Laboratoire de Référence des Mycobactéries,undefined

[9] Institut Pasteur,undefined

[10] 25 rue du Dr. Roux,undefined

[11] 75015 Paris,undefined

[12] France,undefined

[13] Laboratoire d'Immunogénétique Humaine,undefined

[14] Institut Pasteur,undefined

[15] 25 rue du Dr. Roux,undefined

[16] 75015 Paris,undefined

[17] France,undefined

[18] ATelier de Génomique Cognitive,undefined

[19] CNRS ESA 8071/Génopole®,undefined

[20] 523 Terrasses de l'Agora,undefined

[21] 91000 Evry,undefined

[22] France,undefined

[23] Unité d'Immunophysiologie et Parasitisme Intracellulaire,undefined

[24] Institut Pasteur,undefined

[25] 25 rue du Dr. Roux,undefined

[26] 75015 Paris,undefined

[27] France,undefined

来源：

Mammalian Genome | 2003年 / 14卷

关键词：

Inbred Mouse; Consensus Motif; Inbred Mouse Strain; Trapping Strategy; Charged Substrate;

D O I：

暂无

中图分类号：

学科分类号：

摘要：

The P-type ATPases comprise a well-studied family of proteins involved in the active transport of charged substrates across biological membranes. Starting from a mouse bone marrow-derived macrophage cDNA library and using a signal peptide trapping strategy, we identified a new P-type ATPase family member. We characterized the genomic structure of this gene, named Atp10d, as well as its human counterpart. The presence of P-type ATPase consensus motifs and phylogenetic analysis showed that this gene is a member of the type IV, putative amphipath transporters subfamily. We showed that this gene is expressed in kidney and placenta. We also found that the C57BL/6 strain carries a constitutive stop codon in the sequence of Atp10d exon 12, whereas 14 other inbred mouse strains show an uninterrupted reading frame at this location. This mutation in C57BL/6 should lead to a non-functional protein, suggesting that this gene may not be essential. We discuss the involvement of the Atp10d gene in the fat-prone phenotype of the C57BL/6 strain and its physical mapping within a QTL associated with HDL-cholesterol levels.

引用

页码：21 / 30

页数：9