Reference gene selection for real-time RT-PCR normalization in rice field eel (Monopterus albus) during gonad development

被引：0
作者：
Qing Hu
Wei Guo
Yu Gao
Rong Tang
Dapeng Li
机构：
[1] Huazhong Agricultural University,College of Fisheries
[2] Hunan University of Arts and Science,Life Science College
[3] Freshwater Aquaculture Collaborative Innovation Center of Hubei Province,Key Laboratory of Freshwater Animal Breeding
[4] Ministry of Agriculture,undefined
来源：
Fish Physiology and Biochemistry | 2014年 / 40卷
关键词：
Reference gene; Real-time RT-PCR; Stability;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Real-time reverse transcriptase (RT) polymerase chain reaction (PCR) requires data normalization using an appropriate reference gene in order to obtain more reliable results with biological significance. We cloned a partial sequence of elongation factor-1-α (EF1α) and ribosomal protein L17 (RPL17) from Monopterus albus. We investigated the suitability of five commonly used reference genes [18S ribosomal RNA (18S), cytoskeletal protein (β-actin), glyceraldehyde phosphate dehydrogenase (GAPDH), EF1α and RPL17] as potential quantitative reference genes for normalizing real-time RT-PCR data generated in gonads of different developmental stages and in other tissues of M. albus. Analysis of the data indicated that 18S, β-actin and GAPDH are not suitable as reference genes because of their levels of variations of expression. EF1α and RPL17 might be suitable as reference genes in the gonads of different developmental stages as well as in other tissues of M. albus.
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页码：1721 / 1730
页数：9
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