Phylogenetic analysis of porcine reproductive and respiratory syndrome virus in Vietnam, 2021

被引:0
作者
Ngoc Hai Nguyen
Hoang Anh Thu Tran
Trung Quan Nguyen
Phuong Binh Thi Nguyen
Thu Ha Thi Le
Danh Cong Lai
Minh Nam Nguyen
机构
[1] Nong Lam University,Department of Infectious Disease and Veterinary Public Health, Faculty of Animal Husbandry and Veterinary Medicine
[2] Linh Trung Ward,Department of Biomedical Engineering, School of Medicine
[3] Thu Duc City,Research Center for Genetics and Reproductive Health (CGRH), School of Medicine
[4] P&Y Livestock Service Company Limited,undefined
[5] MSD Animal Health Vietnam Company,undefined
[6] Vietnam National University Ho Chi Minh City (VNU-HCM),undefined
[7] National University-HCMC,undefined
[8] Vietnam National University Ho Chi Minh City (VNU-HCM),undefined
来源
Virus Genes | 2022年 / 58卷
关键词
PRRSV; Vaccine; ORF5; Genetic diversity; phylogenetic analysis;
D O I
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学科分类号
摘要
The porcine reproductive and respiratory syndrome virus (PRRSV) causes more economic losses in the swine industry than any other virus. This study aimed to investigate the genetic diversity of PRRSV to assist in evaluating the effectiveness of PRRS vaccines. Twenty-eight samples from clinical cases were collected from 19 farms in seven provinces of Vietnam in 2021. Full-length PRRSV ORF5 genes from the 19 samples were amplified, sequenced, and compared to the corresponding sequences of referenced PRRSV strains from Genbank. The genetic analysis showed that 12 isolates were the highly pathogenic PRRSV subtype (HP—PRRSV) lineage 8, sublineage 8.7; six isolates were the classical North American PRRSV subtype (US-PRRSV), NADC-like group, lineage 1, sublineage 1.4, which were reported in Vietnam for the first time; and the final isolate was a vaccine-like strain. The field isolates of HP-PRRSV had relatively higher genetic diversity with US-PRRSV vaccine strains (84.0–94.5%) than HP-PRRSV vaccine strains (95.3–98.6%). Meanwhile, the six NADC-like isolates had low nucleotide similarity with US-PRRSV and HP-PRRSV vaccine strains (83.4–85.4% and 83.2–84.0%, respectively). Many amino acid substitutions were found in antigenic regions of GP5 involved in response to early antibody production, neutralizing antibodies, and viral immune evasion between these field strains and PRRSV vaccine strains. These findings provide insights into the molecular characteristics, genetic diversity, antigenicity, and evolution of PRRSV strains in Vietnam and postulate a compelling explanation for the limitations of current vaccination efforts.
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页码:361 / 366
页数:5
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