Evaluation of L929 cell morphology on anthocyanin-containing gelatin-based hydrogel for early detection of infection

被引：0
作者：
Ghazal Shineh
Soheila S. Kordestani
Mohammadreza Tahriri
Lobat Tayebi
机构：
[1] Amirkabir University of Technology,Biomaterials Department, Faculty of Biomedical Engineering
[2] Marquette University School of Dentistry,undefined
来源：
Bio-Design and Manufacturing | 2019年 / 2卷
关键词：
Wound healing; Hydrogel; Gelatin; Infection; Anthocyanin; pH;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Wounds heal through a complex process including four phases. Any interruption or interference in healing process can prevent wound healing. Infection is one of the most common complications preventing wounds from healing. In this study, we investigated the fibroblast cell morphology and behavior of a gelatin-based hydrogel containing anthocyanin to determine whether it can be used for early detection of infection. The experimental results ascertained that 6 h after culturing the fibroblasts, they could be well attached to the surface of the hydrogel, and 48 h after seeding, they were spread over the surface and connected with each other. After the addition of a low dose of anthocyanin, the vitality of the cell increased, and the results of the ATR-FTIR analysis showed that anthocyanin could eliminate remaining glutaraldehyde free radicals. Anthocyanin also could change its color after the addition of bacterial supernatant. Thus, hydrogels containing black carrot anthocyanin may be a potential therapeutic and diagnostic strategy to promote wound healing and early detection of infection.
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页码：181 / 186
页数：5
相关论文
共 103 条
[11]  
Baaijens FPT(2010)Temperature and pH affect the production of bacterial biofilm Folia Microbiol 55 75-538
[12]  
Ayati Najafabadi SA(2001)Isolation and determination of anthocyanins in seed coats of black soybean ( J Agric Food Chem 49 5848-7271
[13]  
Shirazaki P(2005) (L.) Merr.) Pol J Food Nutr Sci 14 55-2812
[14]  
Zargar Kharazi A(2005)Acylated anthocyanins as stable, natural food colorants–a review Asian J Biotechnol 9 436-1692
[15]  
Varshosaz J(2017)Flavonoids as antioxidants: determination of radical-scavenging efficiencies Food Nutr Res 61 1361779-486
[16]  
Tahriri M(2008)Anthocyanidins and anthocyanins: colored pigments as food, pharmaceutical ingredients, and the potential health benefits Phytochem Rev 7 281-18184
[17]  
Tayebi L(2017)Anthocyanins: from plant to health Food Anal Methods 10 529-1177
[18]  
Grice EA(2004)Green extraction processes focusing on maximization of black carrot anthocyanins along with cytotoxic activities J Agric Food Chem 52 7264-420
[19]  
Segre JA(2009)Inhibition of cancer cell proliferation in vitro by fruit and berry extracts and correlations with antioxidant levels Food Chem Toxicol 47 2806-undefined
[20]  
Percival SL(2018)Anthocyanins from black soybean seed coats stimulate wound healing in fibroblasts and keratinocytes and prevent inflammation in endothelial cells AAPS PharmSciTech 19 1681-undefined
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