Expression of Channelrhodopsin-2 Using in Suspension Electroporation for Studying the Monosynaptic Transmission in Neuronal Culture

被引：1

作者：

Bal N. ^{[1
]}

Malyshev A. ^{[1
]}

Smirnov I. ^{[1
]}

Balaban P. ^{[1
]}

机构：

[1] Institute of Higher Nervous Activity and Neurophysiology of RAS, 5A Butlerova St., Moscow

来源：

BioNanoScience | 2016年 / 6卷 / 04期

基金：

俄罗斯基础研究基金会; 俄罗斯科学基金会;

关键词：

Channelrhodopsin; Electroporation; Neuronal culture; Optogenetics; Synaptic transmission;

D O I：

10.1007/s12668-016-0228-7

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Understanding the mechanism of synaptic transmission and its plasticity is one of the central goals of modern neuroscience. Neuronal culture is a very convenient model for studying mechanisms of synaptic transmission. However, investigation of two or more synaptically connected neurons in culture by conventional methods is a difficult task. In this study, we describe new protocol for studying the synaptic transmission between cultured neurons using in suspension electroporation for the expression of channelrhodopsin-2 (ChR2) which was applied to only certain subpopulation of cultured cells, leaving the majority of neurons completely untreated. We show that this technique allows reliable long-lasting (hours) recording of monosynaptic excitatory postsynaptic potentials (EPSPs) in cultured hippocampal neurons using a repeated light stimulation of neighboring ChR2-expressing neurons. © 2016, Springer Science+Business Media New York.

引用

页码：329 / 331

页数：2

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