Phenolic composition and neuroprotective effects of the ethyl-acetate fraction from Inonotus sanghuang against H2O2-induced apoptotic cell death of primary cortical neuronal cells

被引：0
作者：
Kun Liu
Cuilian Qi
Yihang Liu
Yaping Huai
Huagang Hu
Xuan Xiao
Junpeng Wang
机构：
[1] Hebei University of Economics and Business,College of Biology Science and Engineering
[2] Hebei University of Economics and Business,Department of Mathematics and Statistics
[3] Wenzhou-Kean University,Department of Computer Science
[4] Shenzhen Longhua District Central Hospital,Department of Rehabilitation
[5] Beijing Xiaotangshan Hospital,Chronic Disease Rehabilitation Laboratory of Integrated Traditional Chinese and Western Medicine
[6] Henan University,Institute of Infection and Immunity of Huaihe Hospital
来源：
Food Science and Biotechnology | 2022年 / 31卷
关键词：
Neuroprotective effect; Anti-inflammatory; Antioxidation; Anti-apoptosis;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
The study aimed to characterize phenolic compounds of the Inonotus sanghuang’s ethyl-acetate fraction (EAF) and assess the neuroprotective effect of EAF using the H2O2-treated primary cortical neuronal cells (PCNC) model. Using HPLC-ECD, 5 phenolics were identified and quantified from EAF. H2O2-treated PCNC experiments in vitro showed that pretreatment with EAF increased the GSH-PX and SOD activities and reduced the NO, MDA, and Aβ contents. Furthermore, EAF suppressed the production of IL-1β, IFN-γ, IL-6, and TNF-α in H2O2-treated PCNC. Other mechanisms found that EAF reduced Bax, caspase 9, and caspase 3 expressions at the mRNA and protein levels while increasing Bcl-2 expression at the mRNA and protein levels. These results showed that EAF could serve as potential agents for anti-NDD.
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页码：1213 / 1223
页数：10
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