Cloning and sequence analysis of the glyceraldehyde-3-phosphate dehydrogenase gene from the zygomycetes fungus Rhizomucor miehei

被引：0
作者：
Mária Vastag
Zsolt Kasza
Klára Ács
Tamás Papp
Helmut Schwab
Csaba Vágvölgyi
机构：
[1] Faculty of Sciences,Department of Microbiology
[2] University of Szeged,Institut für Biotechnologie
[3] Technische Universität Graz,undefined
来源：
Antonie van Leeuwenhoek | 2004年 / 86卷
关键词：
Cloning; glyceraldehyde-3-phosphate dehydrogenase gene; polymerase chain reaction;
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摘要：
Rhizomucor miehei is important from a biotechnological aspect in consequence of its content of aspartic proteinase, which has high milk-clotting activity. A genomic library of R. miehei NRRL 5901 has been constructed in a phage (Lambda Fix II) vector. The glyceraldehyde-3-phosphate dehydrogenase (gpd) gene was isolated from this library by hybridization of the recombinant phage clones with a gpd-specific gene probe generated by polymerase chain reaction. The complete nucleotide sequence encodes a putative polypeptide chain of 336 amino acids interrupted by 5 introns. The predicted amino acid sequence of this gene shows a high degree of sequence similarity to the glyceraldehyde-3-phosphate dehydrogenase proteins from yeast and filamentous fungi. The promoter region, containing a consensus TATA box, and 246-bp downstream from the putative stop codon were also determined. The possibility of using the gpd promoter in the construction of new transformation vectors is discussed.
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页码：111 / 119
页数：8
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