Alternative reverse genetics system for influenza viruses based on a synthesized swine 45S rRNA promoter

被引：0
作者：
Kai Wang
Qi Huang
Zhiwei Yang
Kezong Qi
Hongmei Liu
Hongjun Chen
机构：
[1] Shanghai Veterinary Research Institute,College of Animal Science and Technology
[2] CAAS,Animal Influenza Virus Ecology and Pathogenesis Innovation Team
[3] Anhui Agricultural University,undefined
[4] The Agricultural Science and Technology Innovation Program,undefined
来源：
Virus Genes | 2017年 / 53卷
关键词：
Influenza virus; Swine; 45 s rRNA promoter; Reverse genetics; Rescue;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
We generated an alternative reverse genetics (RG) system based on a synthesized swine 45S rRNA promoter to rescue the H3N2 subtype swine influenza virus. All eight flanking segment cassettes of A/swine/Henan/7/2010 (H3N2) were amplified with ambisense expression elements from RG plasmids. All segments were then recombined with the pHC2014 vector, which contained the synthesized swine 45S rRNA promoter (spol1) and its terminal sequence (t1) in a pcDNA3 backbone. As a result, we obtained a set of RG plasmids carrying the corresponding eight-segment cassettes. We efficiently generated the H3N2 virus after transfection into 293T/PK15, PK15, and 293T cells. The efficiency of spol1-driven influenza virus rescue in PK15 cells was similar to that in 293T cells by titration using the human pol1 RG system. Our approach suggests that an alternative spol1-based RG system can produce influenza viruses.
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页码：661 / 666
页数：5
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