Molecular assembly and structure of the bacteriophage T4 tail

被引：23

作者：

Arisaka F. ^{[1
]}

Yap M.L. ^{[2
]}

Kanamaru S. ^{[3
]}

Rossmann M.G. ^{[2
]}

机构：

[1] Life Science Research Center, School of Bioresource Science, Nihon University, 1866 Kameino, Fujisawa

[2] Department of Biological Sciences, Purdue University, West Lafayette, 47907, IN

[3] Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, 4259, Nagatsuta, Midori-ku, Yokohama

来源：

Biophysical Reviews | 2016年 / 8卷 / 4期

基金：

美国国家卫生研究院; 日本学术振兴会;

关键词：

Assembly; Bacteriophage; Contractile tail; Infection; Molecular recognition; Tail baseplate;

D O I：

10.1007/s12551-016-0230-x

中图分类号：

学科分类号：

摘要：

The tail of bacteriophage T4 undergoes large structural changes upon infection while delivering the phage genome into the host cell. The baseplate is located at the distal end of the contractile tail and plays a central role in transmitting the signal to the tail sheath that the tailfibers have been adsorbed by a host bacterium. This then triggers the sheath contraction. In order to understand the mechanism of assembly and conformational changes of the baseplate upon infection, we have determined the structure of an in vitro assembled baseplate through the three-dimensional reconstruction of cryo-electron microscopy images to a resolution of 3.8 Å from electron micrographs. The atomic structure was fitted to the baseplate structure before and after sheath contraction in order to elucidate the conformational changes that occur after bacteriophage T4 has attached itself to a cell surface. The structure was also used to investigate the protease digestion of the assembly intermediates and the mutation sites of the tail genes, resulting in a number of phenotypes. © 2016, International Union for Pure and Applied Biophysics (IUPAB) and Springer-Verlag Berlin Heidelberg.

引用

页码：385 / 396

页数：11

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