Rapid cold hardening response of the phytoseiid mite Neoseiulus striatus: increased cold tolerance but not reduced predation

The predatory mite Neoseiulus striatus (Wu) (Acari: Phytoseiidae), which has been found on maize plants in Inner Mongolia, is regarded as a promising biological control agent of small sucking pests, especially Tetranychid mites. Temperature is an important abiotic factor, and a sudden drop in temperature may affect its performance when released in areas with big circadian temperature differences. Rapid cold hardening is a type of phenotypic plasticity that allows ectotherms to quickly enhance their cold tolerance in response to a brief chilling exposure. However, it is not clear whether N. striatus possesses such plasticity. To understand how this species of phytoseiid mite copes with short-term low-temperature stress, its rapid cold hardening response was studied in the laboratory by first exploring its critical temperature. Then, the effects of exposure to a series of temperatures (0, 5, 10, 15, and 20 °C) for different durations on the survival of N. striatus were investigated to determine the optimal temperature and duration for cold hardening. Moreover, the effect of cold hardening on the consumption of Tetranychus urticae by N. striatus was also investigated, as was the response of immature stages. The critical temperature for N. striatus was − 15 °C, at which its survival dropped below 10%. An exposure to 5 °C for 2 h was optimal for rapid cold hardening, efficiently increasing survival in N. striatus. Rapid cold hardening was found in all developmental stages of N. striatus, and there was no significant effect of exposure on the consumption of spider mites. Our study shows that exposure to low temperatures during a limited period can enhance the cold hardiness but not reduce predation by N. striatus, which will be advantageous to its field applications during a period of large diurnal temperature fluctuations.