Reactivation of sarin-inhibited pig brain acetylcholinesterase using oxime antidotes.

被引：7
作者：
Kuca K. [1 ]
Jun D. [1 ]
机构：
[1] Centre of Advanced Studies, Department of Toxicology, Faculty Military Health Sciences, University of Defence, Hradec Kralove
来源：
Journal of Medical Toxicology | 2006年 / 2卷 / 4期
关键词：
acetylcholinesterase; reactivation; sarin; nerve agents; inhibition; pig brain homogenate;
D O I：
10.1007/BF03161181
中图分类号：
学科分类号：
摘要：
INTRODUCTION: Organophosphorus nerve agents inhibit the enzyme, acetylcholinesterase (AChE; EC 3.1.1.7). AChE reactivators (also known as oximes) are generally used for the reactivation of an inhibited enzyme. METHODS: Two new AChE reactivators--K033 and K027--were tested for their in vitro reactivation of sarin-inhibited pig-brain AChE. Their reactivation potencies were compared with the commercially available AChE reactivators, pralidoxime, obidoxime, and HI-6. RESULTS: Of the oximes tested, the newly developed oxime K027 achieved the highest reactivation potency (100%; concentration of the oxime -10(-2) M). However, oxime HI-6 (33%) and obidoxime (23%) seem to be the best AChE reactivators for human relevant doses (10(-4) M and lower). CONCLUSION: For human relevant doses, newly developed oximes (K027 and K033) do not surpass the reactivation potency of the most promising oxime, HI-6.
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页码：141 / 146
页数：5
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