KLF5 regulated lncRNA RP1 promotes the growth and metastasis of breast cancer via repressing p27kip1 translation

被引：72

作者：

Jia, Xiaoting ^{[1
]}

Shi, Lejuan ^{[1
]}

Wang, Xiaorong ^{[1
]}

Luo, Liyun ^{[1
]}

Ling, Li ^{[1
]}

Yin, Jiang ^{[1
]}

Song, Ying ^{[1
]}

Zhang, Zhijie ^{[1
]}

Qiu, Ni ^{[1
]}

Liu, Hao ^{[1
]}

Deng, Min ^{[1
]}

He, Zhimin ^{[1
]}

Li, Hongsheng ^{[1
]}

Zheng, Guopei ^{[1
]}

机构：

[1] Guangzhou Med Univ, Affiliated Canc Hosp & Inst, Key Lab Prot Modificat & Degradat, State Key Lab Resp,Guangzhou Key Lab Translat Med, Hengzhigang Rd 78, Guangzhou 510095, Guangdong, Peoples R China

来源：

CELL DEATH & DISEASE | 2019年 / 10卷 / 5期

基金：

中国国家自然科学基金;

关键词：

LONG NONCODING RNA; CELL-PROLIFERATION; GASTRIC-CANCER; EXPRESSION; BINDING; DOWNSTREAM; INHIBITION; TRANSITION; P27(KIP1); UPSTREAM;

D O I：

10.1038/s41419-019-1566-5

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Increasing evidence suggest that lncRNAs (long noncoding RNAs) play important roles in human cancer. Breast cancer is a heterogeneous disease and the potential involvement of lncRNAs in breast cancer remains unexplored. In this study, we characterized a novel lncRNA, RP7-506.5 (termed as RP1). We found that RP1 was highly expressed in breast cancer and predicted poor prognosis of breast cancer patients. Gain-of-function and loss-of-function assays showed that RP1 promoted the proliferation and metastasis of breast cancer cells in vitro and in vivo. Mechanistically, RP1 maintained the EMT and stemness states of breast cancer cells via repressing p27kip1 protein expression. RP1 combined with the complex p-4E-BP1/eIF4E to prevent eIF4E from interacting with eIF4G, therefore attenuating the translational efficiency of p27kip1 mRNA. Furthermore, we found that p27kip1 evidently downregulated Snail1 but not ZEB1 to inhibit invasion of breast cancer cells. Kruppel-like factor 5 (KLF5) was positively correlated with RP1 in breast cancer tissues. Moreover, we demonstrated that KLF5 recruited p300 to the RP1 promoter to enhance RP1 expression. Taken together, our findings demonstrated that KLF5-regulated RP1 plays an oncogenic role in breast cancer by suppressing p27kip1, providing support for the clinical investigation of therapeutic approaches focusing on RP1.

引用

页数：16

共 41 条

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