Circadian clock components in the rat neocortex: daily dynamics, localization and regulation

被引:0
作者
Martin F. Rath
Kristian Rohde
Jan Fahrenkrug
Morten Møller
机构
[1] Department of Neuroscience and Pharmacology,
[2] Faculty of Health and Medical Sciences,undefined
[3] University of Copenhagen,undefined
[4] Panum Institute 24.2,undefined
[5] Department of Clinical Biochemistry,undefined
[6] Faculty of Health and Medical Sciences,undefined
[7] University of Copenhagen,undefined
[8] Bispebjerg Hospital,undefined
来源
Brain Structure and Function | 2013年 / 218卷
关键词
Cerebral cortex; Circadian rhythm; Clock gene; Peripheral oscillator; Suprachiasmatic nucleus;
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学科分类号
摘要
The circadian master clock of the mammalian brain resides in the suprachiasmatic nucleus (SCN) of the hypothalamus. At the molecular level, the clock of the SCN is driven by a transcriptional/posttranslational autoregulatory network with clock gene products as core elements. Recent investigations have shown the presence of peripheral clocks in extra-hypothalamic areas of the central nervous system. However, knowledge on the clock gene network in the cerebral cortex is limited. We here show that the mammalian clock genes Per1, Per2, Per3, Cry1, Cry2, Bmal1, Clock, Nr1d1 and Dbp are expressed in the rat neocortex. Among these, Per1, Per2, Per3, Cry1, Bmal1, Nr1d1 and Dbp were found to exhibit daily rhythms. The amplitude of circadian oscillation in neocortical clock gene expression was damped and the peak delayed as compared with the SCN. Lesions of the SCN revealed that rhythmic clock gene expression in the neocortex is dependent on the SCN. In situ hybridization and immunohistochemistry showed that products of the canonical clock gene Per2 are located in perikarya throughout all areas of the neocortex. These findings show that local circadian oscillators driven by the SCN reside within neurons of the neocortex.
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页码:551 / 562
页数:11
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