Biochemical and Structural Characterization of a Detergent Stable Alkaline Serine Keratinase from Paenibacillus Woosongensis TKB2: A Potential Additive for Laundry Detergent

被引:0
作者
Tanmay Paul
Arpan Das
Arpita Mandal
Suman K. Halder
Pradeep Kumar DasMohapatra
Bikas R. Pati
Keshab Chandra Mondal
机构
[1] Vidyasagar University,Department of Microbiology
[2] Raja N.L. Khan Women’s College,Department of Microbiology
来源
Waste and Biomass Valorization | 2014年 / 5卷
关键词
Monomeric; Keratinase; Circular dichroism; Detergents; Febric;
D O I
暂无
中图分类号
学科分类号
摘要
A monomeric alkaline keratinolytic serine protease with a molecular weight 190.24 kDa was purified from Paenibacillus woosongensis TKB2 in submerged fermentation using waste chicken feather as substrate. The purified keratinase was highest activity at pH 9.0 and 50 °C, requiring Mo+ for increasing fourfold enzyme activities, showed substrate specificity for keratin powder. The Km and Vmax for the enzyme was 1.4 mg/ml and 251.1 U/ml respectively. An initial analysis of the circular dichroism spectrum in the ultraviolet range revealed that the protease is predominantly an α-helix structure. In the presence of 7 mg/ml (w/v) detergents, the protease was active and retained 40–90 % activity. Therefore, it may have a possible application in laundry formulations. The keratinase combined with detergent was able to destain blood, fruit juice and turmeric stained cloth within 30 min without damaging the fabric structure and strength.
引用
收藏
页码:563 / 574
页数:11
相关论文
共 105 条
[21]  
Price NC(2003) A21: purification and characterization Enzyme Microb. Technol. 32 519-524
[22]  
Paul T.(2010)A novel serine metallokeratinase from a newly isolated Appl. Microbiol. Biotechnol. 85 1735-1750
[23]  
Halder S.K.(2010) A1 grown on chicken feather meal: biochemical and molecular characterization Bioresour. Technol. 101 8361-8369
[24]  
Das A.(2005)Bacterial alkaline proteases: molecular approaches and industrial applications Ind. J. Biochem. Biophys. 42 100-105
[25]  
Bera S.(2011)Detergent proteases Process Biochem. 46 579-585
[26]  
Maity C.(2006)Purification and biochemical characterization of a serine protease inhibitor from Enzyme Microb. Technol. 38 49-54
[27]  
Mandal A.(2001). Seeds: insight into structural and antimalarial features World J. Microbiol. Biotechnol. 17 493-497
[28]  
Das P.S.(undefined)Purification, characterization and secondary structure elucidation of a detergent stable, halotolerant, thermoalkaline protease from undefined undefined undefined-undefined
[29]  
Mohapatra P.K.D.(undefined) SIU1 undefined undefined undefined-undefined
[30]  
Pati B.R.(undefined)Characterization of a Keratinolytic serine protease from undefined undefined undefined-undefined