Synthesis and characterization of fructooligosaccharides using levansucrase with a high concentration of sucrose

被引：0
作者：
Eun-Seong Seo
Jin-Ha Lee
Jae-Young Cho
Mi-Young Seo
Hee-Sun Lee
Seuk-Sang Chang
Hyung-Jong Lee
Jeong-Sik Choi
Doman Kim
机构：
[1] Chonnam National University,Laboratory of Functional Carbohydrate Enzyme and Microbial Genomics
[2] Chonam National University,Department of Material Chemical and Biochemical Engineering
[3] Chonnam National University,Engineering Research institute
[4] Chonnam National University,Department of Physics
[5] Chonnam National University,Department of Molecular and Biotechnology
[6] Chonnam National University,Shool of Biological Sciences and Technology and Research Institute for Catalysis
[7] Pohang Accelerator Laboratory,Biology Research Center for industrial Accelerators
[8] Dongshin University,undefined
来源：
Biotechnology and Bioprocess Engineering | 2004年 / 9卷
关键词：
levansucrase; fructooligosaccharides; inhibition; insoluble glucan;
D O I：
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中图分类号：
学科分类号：
摘要：
A method for synthesizing branched fructo-oligosaccharides (BFOS) with a high concentration of sucrose (1–3 M) was developed using levansucrase prepared fromLeuconostoc mesenteroides B-1355C. The degree of polymerization of oligosaccharides synthesized according to the present method ranged from 2 to over 15. The synthesized BFOS were stable at a pH ranges of 2 to 4 under 120°C. The percentage of BFOS in the reaction digest was 95.7% (excluding monosaccharides; 4.3% was levan). BFOS reduced the insoluble glucan formation byStreptococcus sobrinus on the surfaces of glass vials or stainless steel wires in the presence of sucrose. They also reduced the growth and acid productions ofS. sobrinus. Oligosaccharides can be used as sweeteners for foods such as beverages requiring thermo-and acid-stable properties and as potential inhibitors of dental caries.
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页码：339 / 344
页数：5
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