Maize NDC80 is a constitutive feature of the central kinetochore

被引：0
作者：
Yaqing Du
R. Kelly Dawe
机构：
[1] University of Georgia,Department of Plant Biology
[2] University of Georgia,Department of Genetics
来源：
Chromosome Research | 2007年 / 15卷
关键词：
kinetochore; centromere; NDC80; mitosis; meiosis;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
In yeast and animals, Nuclear Division Cycle 80 (NDC80) is an important kinetochore protein that binds to microtubules and mediates chromosome movement. Its localization pattern is unusual, since it is generally not viewed as either an inner (centromeric chromatin) or outer (regulatory) component of the kinetochore. Here we report the characterization of NDC80 in a higher plant. By taking advantage of the large meiotic kinetochores of maize, we were able to show that NDC80 localizes outside of the constitutive kinetochore protein CENP-C. Further, a detailed analysis of mitosis indicates that NDC80 is stably present on kinetochores throughout the cell cycle. The quantity of NDC80 positively correlates with measured quantities of DNA and CENP-C, suggesting that NDC80 rapidly associates with DNA following replication and is stably maintained at centromeres during cell division. The data suggest that in plants NDC80 is on par with ‘foundation’ kinetochore proteins such as CENH3 and CENP-C.
引用
收藏
页码：767 / 775
页数：8
相关论文
共 137 条
[1]  
Amor DJ(2004)Building the centromere: from foundation proteins to 3D organization Trends Cell Biol 14 359-368
[2]  
Kalitsis P(1982)Two different monoclonal antibodies to tubulin inhibit the bending of reactivated sea urchin spermatozoa Cell Motil 2 599-614
[3]  
Sumer H(2005)Dissociation of the Nuf2-Ndc80 complex releases centromeres from the spindle-pole body during meiotic prophase in fission yeast Mol Biol Cell 16 2325-2338
[4]  
Choo KH(2004)Identification of two novel components of the human NDC80 kinetochore complex J Biol Chem 279 13076-13085
[5]  
Asai DJ(2006)The conserved KMN network constitutes the core microtubule-binding site of the kinetochore Cell 127 983-997
[6]  
Brokaw CJ(1997)HEC, a novel nuclear protein rich in leucine heptad repeats specifically involved in mitosis Mol Cell Biol 17 6049-6056
[7]  
Thompson WC(1994)Meiotic chromosome pairing in maize is associated with a novel chromatin organization Cell 76 901-912
[8]  
Wilson L(1999)A maize homolog of mammalian CENPC is a constitutive component of the inner kinetochore Plant Cell 11 1227-1238
[9]  
Asakawa H(2005)Hec1 and nuf2 are core components of the kinetochore outer plate essential for organizing microtubule attachment sites Mol Biol Cell 16 519-531
[10]  
Hayashi A(2006)Kinetochore microtubule dynamics and attachment stability are regulated by Hec1 Cell 127 969-982
12345678910