Plastic vs. glass: the effect of the synthetic material in contact with DMEK tissue during staining on endothelial cell loss

被引：0

作者：

Al-Kaabi, Abdullah ^{[1
]}

Gustavson, Britta ^{[2
]}

Alotaibi, Abdulrahman ^{[3
]}

Aljohani, Ayman ^{[3
]}

Talajic, Julia ^{[4
]}

Choremis, Johanna ^{[3
,4
]}

机构：

[1] Univ Ottawa, Ophthalmol Dept, Ottawa, ON, Canada

[2] Univ British Columbia, Ophthalmol Dept, Vancouver, BC, Canada

[3] McGill Univ, Ophthalmol Dept, Montreal, PQ, Canada

[4] Univ Montreal, Fac Med, Ophthalmol Dept, CP 6128,Ctr Ville Branch, Montreal, PQ H3C 3J7, Canada

来源：

INTERNATIONAL OPHTHALMOLOGY | 2024年 / 44卷 / 01期

关键词：

DMEK; Endothelial keratoplasty; Corneal transplant; Glass; Plastic; KERATOPLASTY;

D O I：

10.1007/s10792-024-03066-0

中图分类号：

R77 [眼科学];

学科分类号：

100212 ;

摘要：

PurposeEndothelial cell loss (ECL) during Descemet membrane endothelial keratoplasty (DMEK) graft preparation has been shown to affect graft survival and the need for re-grafting. The purpose of this study was to quantitatively assess the impact of the plastic and glass mediums in contact with DMEK donor tissue during intra-operative graft staining on ECL.MethodsRetrospective study that included patients who underwent DMEK surgery between January 2019 and June 2021 at Hopital Maisonneuve-Rosemont and the Jewish General Hospital in Montreal, Canada. DMEK grafts were stained with 0.06% Trypan blue ophthalmic solution (VisionBlue (R), Dutch Ophthalmic, USA, Exeter, NH) for 120 s in either a plastic or glass medium prior to delivery into the recipient's eye. The ECL was compared between the two groups 12-30 months post-operatively.ResultsECL at 12-30 months was significantly less in the eyes that had received grafts stained in a plastic medium compared to those stained in a glass medium. Graft survival and re-bubbling was higher in the glass group however this difference was not statistically significant.ConclusionStaining of the DMEK graft in a plastic medium caused less ECL compared to the glass medium.

引用

页数：8