STED microscopy with continuous wave beams

被引：0

作者：

Katrin I Willig

Benjamin Harke

Rebecca Medda

Stefan W Hell

机构：

[1] Max Planck Institute for Biophysical Chemistry,Department of NanoBiophotonics

[2] Am Fassberg 11,undefined

来源：

Nature Methods | 2007年 / 4卷

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摘要：

We report stimulated emission depletion (STED) fluorescence microscopy with continuous wave (CW) laser beams. Lateral fluorescence confinement from the scanning focal spot delivered a resolution of 29–60 nm in the focal plane, corresponding to a 5–8-fold improvement over the diffraction barrier. Axial spot confinement increased the axial resolution by 3.5-fold. We observed three-dimensional (3D) subdiffraction resolution in 3D image stacks. Viable for fluorophores with low triplet yield, the use of CW light sources greatly simplifies the implementation of this concept of far-field fluorescence nanoscopy.

引用

页码：915 / 918

页数：3