STED microscopy with continuous wave beams

被引:0
作者
Katrin I Willig
Benjamin Harke
Rebecca Medda
Stefan W Hell
机构
[1] Max Planck Institute for Biophysical Chemistry,Department of NanoBiophotonics
[2] Am Fassberg 11,undefined
来源
Nature Methods | 2007年 / 4卷
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摘要
We report stimulated emission depletion (STED) fluorescence microscopy with continuous wave (CW) laser beams. Lateral fluorescence confinement from the scanning focal spot delivered a resolution of 29–60 nm in the focal plane, corresponding to a 5–8-fold improvement over the diffraction barrier. Axial spot confinement increased the axial resolution by 3.5-fold. We observed three-dimensional (3D) subdiffraction resolution in 3D image stacks. Viable for fluorophores with low triplet yield, the use of CW light sources greatly simplifies the implementation of this concept of far-field fluorescence nanoscopy.
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页码:915 / 918
页数:3
相关论文
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