Sequence Dependent Femtosecond-Resolved Hydration Dynamics in the Minor Groove of DNA and Histone—DNA Complexes

被引：0
作者：
Debapriya Banerjee
Abhinandan Makhal
Samir Kumar Pal
机构：
[1] S. N. Bose National Centre for Basic Sciences,Unit for Nano Science & Technology, Department of Chemical, Biological & Macromolecular Sciences
来源：
Journal of Fluorescence | 2009年 / 19卷
关键词：
Hoechst; Sequence dependent molecular recognition; Femtosecond-resolved hydration dynamics;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Understanding the sequence dependent molecular recognition of DNA is crucial for the rational design of many drugs. Femtosecond resolved studies on the hydration dynamics of the dodecamer duplexes having sequences (CGCGAATTCGCG)2 and (CGCAAATTTGCG)2, and their complexes with the nucleic protein histone 1 (H1) reveal significant correlation of the molecular recognition of the DNA and DNA-protein complexes with the dynamics of hydration. The different molecular recognition of DNA and DNA-protein complexes is also borne out by circular dichroism (CD) and fluorescence detected CD measurements.
引用
收藏
页码：1111 / 1118
页数：7
相关论文
共 94 条
[1]  
Pjura PE(1987)Binding of Hoechst 33258 to the minor groove of B-DNA J Mol Biol 197 257-271
[2]  
Grzeskowiak K(1992)Molecular structure of the B-DNA dodecamer d(CGCAAATTTGCG) J Mol Biol 226 1161-1173
[3]  
Dickerson RE(1997) An examination of propeller twist and minor-groove water structure at 2·2 Å resolution Biochim Biophys Acta 1350 205-220
[4]  
Edwards KJ(1997)Hydration and solution structure of d(CGCAAATTTGCG) J Mol Biol 271 244-257
[5]  
Brown DG(2005)and its complex with propamidine from NMR and molecular modelling Biochemistry 44 9785-9794
[6]  
Spink N(2007)Specific binding of Hoechst 33258 to the d(CGCAAATTTGCG) J Mol Biol 365 1-9
[7]  
Skelly JV(2003) duplex: calorimetric and spectroscopic studies Proc Natl Acad Sci USA 100 8113-8118
[8]  
Neidle S(2004)Association of the minor groove binding drug Hoechst 33258 with d(CGCGAATTCGCG) Chem Rev 104 2099-2123
[9]  
Lane AN(1999): volumetric, calorimetric, and spectroscopic characterizations Biochemisty 38 16443-16451
[10]  
Jenkins TC(1991)What drives proteins into the major or minor grooves of DNA? Biochemistry 30 10294-10306
12345678910