Cloning and characterization of pea apyrases: involvement of PsAPY1 in response to signal molecules from the pea pathogen Mycosphaerella pinodes

被引：15

作者：

Tomoharu Kawahara

Kazuhiro Toyoda

Akinori Kiba

Akiko Miura

Takako Ohgawara

Mikihiro Yamamoto

Yoshishige Inagaki

Yuki Ichinose

Tomonori Shiraishi

机构：

[1] Laboratory of Plant Pathology and Genetic Engineering,

[2] College of Agriculture,undefined

[3] Okayama University,undefined

[4] Okayama 700-8530,undefined

[5] Japan,undefined

来源：

Journal of General Plant Pathology | 2003年 / 69卷 / 1期

关键词：

Key words Apyrases (NTPases); Biological stresses; Elicitor; Mycosphaerella pinodes; Pisum sativum L.; Suppressor;

D O I：

10.1007/s10327-002-0003-1

中图分类号：

学科分类号：

摘要：

Two nucleoside triphosphatase (NTPase) cDNA clones were isolated from a cDNA library of Pisum sativum L., cv. Midoriusui. The genes encoding the cDNAs were designated PsAPY1 and PsAPY2. PsAPY1 included the N-terminal amino acid sequence of an NTPase bound to pea cell wall. The phylogenic analysis indicated that PsAPY1 belongs to an NTPase subfamily responsive to environmental stimuli and that PsAPY2 belongs to a discrete subfamily, the physiological role of which is almost unknown. The adenosine triphosphatase activity of recombinant PsAPY1 was regulated by an elicitor and a suppressor from the pea pathogen Mycosphaerella pinodes. Based on these findings, we discuss the role of NTPases in response to biological stresses.

引用

页码：33 / 38

页数：5