Controlled Growth of Chinese Hamster Ovary Cells and High Expression of Antibody-IL-2 Fusion Proteins by Temperature Manipulation

被引：3
作者：
Ming Shi
Zhigang Xie
Ming Yu
Beifen Shen
Ning Guo
机构：
[1] Institute of Basic Medical Sciences,Department of Molecular Immunology
来源：
Biotechnology Letters | 2005年 / 27卷
关键词：
antibody; cell cycle; fusion protein; gene expression; Interleukin 2; low temperature;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Growth and the expression of the anti-ErbB2 scFv-Fc-IL2 fusion protein in Chinese hamster ovary (CHO) cells were in association at 37 °C. The expression of the fusion protein was no more than 25 μg/ml. At 30 °C the cell growth was arrested but the cells continued to produce the fusion protein up to 60–80 μg/ml. About 50% of CHO cells were rapidly blocked in G2/M phase after the temperature was shifted from 37 to 30 °C. Lowering temperature resulted in cell growth arrest, but maintained cell viability for a longer time and enhanced the production of the antibody-IL-2 fusion protein in CHO cells.
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页码：1879 / 1884
页数：5
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