Induction of hairy root cultures of Azadirachta indica A. Juss. and their production of azadirachtin and other important insect bioactive metabolites

被引：0

作者：

E. Allan

J. Eeswara

A. Jarvis

A. Mordue (Luntz)

E. Morgan

T. Stuchbury

机构：

[1] Department of Agriculture and Forestry,

[2] University of Aberdeen,undefined

[3] MacRobert Building,undefined

[4] 581 King Street,undefined

[5] Aberdeen,undefined

[6] AB24 5UA,undefined

[7] UK,undefined

[8] Department of Chemistry,undefined

[9] Keele University,undefined

[10] Staffordshire,undefined

[11] ST5 5BG,undefined

[12] UK,undefined

[13] Department of Zoology,undefined

[14] University of Aberdeen,undefined

[15] Tillydrone Avenue,undefined

[16] Aberdeen,undefined

[17] AB24 2TZ,undefined

[18] UK,undefined

来源：

Plant Cell Reports | 2002年 / 21卷

关键词：

Azadirachta indica Neem Azadirachtin Hairy root cultures;

D O I：

暂无

中图分类号：

学科分类号：

摘要：

Hairy root cultures were established from stem and leaf explants of Azadirachta indica A. Juss (neem) following infection with Agrobacterium rhizogenes. Transformation was confirmed using polymerase chain reaction analysis. The cultures had a relatively fast growth rate (0.134 g dry weight day–1) and showed a 100-fold increase in biomass over a 4-week culture period. Both the roots and cultivation medium were antifeedant as determined by a no-choice bioassay using the desert locust, Schistocerca gregaria. Azadirachtin, nimbin, salannin, 3-acetyl-1-tigloylazadirachtinin and 3-tigloylazadirachtol were detected in the hairy roots by supercritical fluid chromatography. The cultivation medium contained 3-acetyl-1-tigloylazadirachtinin, 3-tigloylazadirachtol and desacetylsalannin.

引用

页码：374 / 379

页数：5

共 48 条

[1] Induction of hairy root cultures of Azadirachta indica A.!Juss and their production of azadirachtin and other important insect bioactive metabolites
Allan, EJ
Eeswara, JP
Jarvis, AP
Mordue, AJ
Morgan, ED
Stuchbury, T
PLANT CELL REPORTS, 2002, 21 (04) : 374 - 379
[2] Production of azadirachtin by callus cultures from Azadirachta indica A. Juss.
Wewetzer, A
AZADIRACHTA INDICA A. JUSS., 1999, : 259 - 267
[3] Enhanced production of azadirachtin by hairy root cultures of Azadirachta indica A.!Juss by elicitation and media optimization
Satdive, Ramesh K.
Fulzele, Devanand P.
Eapen, Susan
JOURNAL OF BIOTECHNOLOGY, 2007, 128 (02) : 281 - 289
[4] An endophytic fungus from Azadirachta indica A. Juss. that produces azadirachtin
Souvik Kusari
Vijay C. Verma
Marc Lamshoeft
Michael Spiteller
World Journal of Microbiology and Biotechnology, 2012, 28 : 1287 - 1294
[5] An endophytic fungus from Azadirachta indica A. Juss. that produces azadirachtin
Kusari, Souvik
Verma, Vijay C.
Lamshoeft, Marc
Spiteller, Michael
WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY, 2012, 28 (03): : 1287 - 1294
[6] Somatic embryogenesis in clonal neem, Azadirachta indica A. Juss. and analysis for in vitro azadirachtin production
Chakradhar Akula
Anisha Akula
Rod Drew
In Vitro Cellular & Developmental Biology - Plant, 2003, 39 : 304 - 310
[7] Statistical optimization of media for enhanced azadirachtin production from redifferentiated zygotic embryo cultures of neem (Azadirachta indica A. Juss.)
Mithilesh Singh
Rakhi Chaturvedi
In Vitro Cellular & Developmental Biology - Plant, 2012, 48 : 92 - 98
[8] Cell Line Selection and Media Optimization for Enhanced Azadirachtin Production from In Vitro Cultures of Neem (Azadirachta indica A. Juss.)
Chaturvedi, Rakhi
Singh, Mithilesh
IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL, 2011, 47 : S72 - S72
[9] Statistical optimization of media for enhanced azadirachtin production from redifferentiated zygotic embryo cultures of neem (Azadirachta indica A. Juss.)
Singh, Mithilesh
Chaturvedi, Rakhi
IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT, 2012, 48 (01) : 92 - 98
[10] Bioproduction of azadirachtin-A, nimbin and salannin in callus and cell suspension cultures of neem (Azadirachta indica A. Juss.)
Babu, Vidhu Sankar
Narasimhan, S.
Nair, G. M.
CURRENT SCIENCE, 2006, 91 (01): : 22 - 24

← 1 2 3 4 5 →