Differential Gene Regulation of the Human Blastocyst Trophectoderm and Inner Cell Mass by Progesterone

被引：0
作者：
Kathryn Shaia Snow
Douglas J. Raburn
Thomas M. Price
机构：
[1] Duke University,Division of Reproductive Endocrinology and Infertility Department of Obstetrics and Gynecology
来源：
Reproductive Sciences | 2024年 / 31卷
关键词：
Blastocyst gene expression; Embryo culture media; RNA-seq; P4 supplementation;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Knowledge of action of progesterone (P4) on the human preimplantation embryo is lacking. The objective of this study was to determine expression of a mitochondrial P4 receptor (PR-M) in the trophectoderm (TE) and the inner cell mass (ICM) of the human blastocyst and to determine P4-induced gene expression during growth from the cleavage to the blastocyst stage. Previously cryopreserved cleavage stage embryos were treated with P4 (10−6 M) or vehicle until blastocyst development. Cells from the TE and the ICM of dissected euploid embryos underwent RNA-seq analysis, while other embryos were used for analysis of nuclear PR (nPR) and PR-M expression.
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页码：1363 / 1372
页数：9
相关论文
共 206 条
[1]  
Koninckx P(1998)Endometriotic disease: the role of peritoneal fluid Hum Reprod Update 4 741-751
[2]  
Kennedy S(2020)Progesterone increases mitochondria membrane potential in non-human primate oocytes and embryos Reprod Sci 27 1206-1214
[3]  
Barlow D(2019)Progesterone receptor membrane component 1 and 2 regulate granulosa cell mitosis and survival through a NFΚB-dependent mechanism† Biol Reprod 100 1571-1580
[4]  
Dai Q(2016)Progesterone receptor membrane component 1 mediates progesterone-induced suppression of oocyte meiotic prophase i and primordial folliculogenesis Sci Rep 6 36869-2236
[5]  
Provost MP(2003)Cloning, expression, and characterization of a membrane progestin receptor and evidence it is an intermediary in meiotic maturation of fish oocytes PNAS 100 2231-673
[6]  
Raburn DJ(2007)The role of xenopus membrane progesterone receptor β in mediating the effect of progesterone on oocyte maturation Mol Endocrinol 21 664-194
[7]  
Price TM(2015)The role of a mitochondrial progesterone receptor (PR-M) in progesterone action Semin Reprod Med 33 185-429
[8]  
Peluso JJ(2012)Structural and functional analysis of domains of the progesterone receptor Mol Cell Endocrinol 348 418-753
[9]  
Pru CA(2013)A truncated progesterone receptor (PR-M) Localizes to the mitochondrion and controls cellular respiration Mol Endocrinol 27 741-467
[10]  
Liu X(2019)A mitochondrial progesterone receptor increase cardia beta-oxidation and remodeling J Endocr Soc 3 446-E399
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