Selection of Yarrowia lipolytica strains with high protein content from yeasts isolated from different marine environments

被引：5
作者：
Chi Z. [1 ]
Wang F. [1 ]
Wang L. [1 ]
Li J. [1 ]
Wang X. [1 ]
机构：
[1] Unesco Chinese Center of Marine Biotechnology, Ocean University of China
来源：
Journal of Ocean University of China | 2007年 / 6卷 / 4期
关键词：
Biolog; Molecular identification; Single cell protein; Y; lipolytica;
D O I：
10.1007/s11802-007-0360-7
中图分类号：
学科分类号：
摘要：
A total of 78 Yarrowia lipolytica yeast strains from seawater, sediments, mud of salterns, the guts of marine fish, and marine algae were obtained. After the crude protein of the yeasts was estimated by the method of Kjehldahl, we found that seven strains of the marine yeasts grown in soy bean cake hydrolysate with 20 g L-1 of glucose for 48 h at 28°C contained more than 41.0 g protein per 100 g of cell dry weight and the cell dry weight was more than 4.4 g per L of the culture. Among them, strain SWJ-1b contained the highest crude protein. The results of Biolog identification and molecular methods further confirmed that they indeed belonged to Y. lipolytica. © 2007 Science Press.
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收藏
页码：360 / 364
页数：4
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