A genome-scale shRNA resource for transgenic RNAi in Drosophila

被引：0

作者：

Ni J.-Q. ^{[1
,5
]}

Zhou R. ^{[1
,5
,6
]}

Czech B. ^{[2
]}

Liu L.-P. ^{[1
,5
]}

Holderbaum L. ^{[1
]}

Yang-Zhou D. ^{[1
]}

Shim H.-S. ^{[1
]}

Tao R. ^{[1
]}

Handler D. ^{[3
]}

Karpowicz P. ^{[1
]}

Binari R. ^{[1
]}

Booker M. ^{[1
]}

Brennecke J. ^{[3
]}

Perkins L.A. ^{[4
]}

Hannon G.J. ^{[2
]}

Perrimon N. ^{[1
]}

机构：

[1] Department of Genetics, Harvard Medical School, Howard Hughes Medical Institute, Boston, MA

[2] Watson School of Biological Sciences, Howard Hughes Medical Institute, Cold Spring Harbor Laboratory, Cold Spring Harbor, NY

[3] Institute of Molecular Biotechnology, Vienna

[4] Pediatric Surgical Research Labs, Massachusetts General Hospital, Harvard Medical School, Boston, MA

[5] Gene Regulation Laboratory and Tsinghua Fly Center, School of Life Sciences and School of Medicine, Tsinghua University, Beijing

[6] Sanford-Burnham Medical Research Institute, San Diego, CA

来源：

Nature Methods | 2011年 / 8卷 / 5期

基金：

欧洲研究理事会;

关键词：

D O I：

10.1038/nmeth.1592

中图分类号：

学科分类号：

摘要：

Existing transgenic RNAi resources in Drosophila melanogaster based on long double-stranded hairpin RNAs are powerful tools for functional studies, but they are ineffective in gene knockdown during oogenesis, an important model system for the study of many biological questions. We show that shRNAs, modeled on an endogenous microRNA, are extremely effective at silencing gene expression during oogenesis. We also describe our progress toward building a genome-wide shRNA resource. © 2011 Nature America, Inc. All rights reserved.

引用

页码：405 / 407

页数：2

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