Sodium chloride-induced spatial and temporal manifestation in membrane stability index and protein profiles of contrasting wheat (Triticum aestivum L.) genotypes under salt stress

被引：11
作者：
Kumar M. [1 ]
Hasan M. [1 ]
Arora A. [2 ]
Gaikwad K. [3 ]
Kumar S. [1 ]
Rai R.D. [1 ]
Singh A. [1 ]
机构：
[1] Division of Biochemistry, Indian Agricultural Research Institute, New Delhi
[2] Division of Plant Physiology, Indian Agricultural Research Institute, New Delhi
[3] National Research Centre on Plant Biotechnology, New Delhi
来源：
Indian Journal of Plant Physiology | 2015年 / 20卷 / 3期
关键词：
Membrane stability index; Protein profiling; Salt stress; Wheat genotypes;
D O I：
10.1007/s40502-015-0157-4
中图分类号：
学科分类号：
摘要：
The impact of salt stress on root and shoot regions of three contrasting varieties of wheat viz., WH 542 (salt-susceptible), KRL 1–4 (moderately salt-tolerant), KRL 210 (salt-tolerant) under different levels of NaCl (0, 150 and 300 mM) and duration (7, 14 and 21 days after treatment) at seedling stage was studied in terms of the relative water content (RWC), membrane stability index (MSI) and protein expression profiles. There was a progressive decline in MSI due to increase in electrolyte leakage at different levels and durations of salt stress induced cell membrane injury. The data indicated that 14 days of stress imposition at 150 mM salt concentration was the right stage of monitoring biochemical and physiological parameters. Differential expression of soluble proteins in these contrasting wheat genotypes revealed their spatial and temporal partitioning during the salt stress, and suggested that the stress-induced proteins may be involved in modulating effects of salt stress on the plant. Based on RWC, MSI and protein profiles of these genotypes, KRL 210 was found to be the least distressed by the stress imposition while WH 542 was the most affected. © 2015, Indian Society for Plant Physiology.
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页码：271 / 275
页数：4
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