Differences in skin test reactions to official and defined antigens in guinea pigs exposed to non-tuberculous and tuberculous bacteria

被引：0
作者：
Leire Fernández-Veiga
Miguel Fuertes
María V. Geijo
Bernat Pérez de Val
Enric Vidal
Lorraine Michelet
María Laura Boschiroli
Alberto Gómez-Buendía
Javier Bezos
Gareth J. Jones
Martin Vordermeier
Ramón A. Juste
Joseba M. Garrido
Iker A. Sevilla
机构：
[1] Basque Research and Technology Alliance (BRTA),Departamento de Sanidad Animal, NEIKER
[2] Campus de la Universitat Autònoma de Barcelona (UAB),Instituto Vasco de Investigación y Desarrollo Agrario
[3] Campus de la UAB,IRTA, Programa de Sanitat Animal, Centre de Recerca en Sanitat Animal (CReSA)
[4] Université Paris-Est,Unitat Mixta d’investigació IRTA
[5] Universidad Complutense de Madrid,UAB en Sanitat Animal, CReSA
[6] Universidad Complutense de Madrid,Laboratoire de Santé Animale, Unité Zoonoses Bactériennes, Agence Nationale de Sécurité Sanitaire de l’alimentation, de l’environnement et du Travail (ANSES)
[7] Animal and Plant Health Agency (APHA),Centro de Vigilancia Sanitaria Veterinaria (VISAVET)
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Scientific Reports | / 13卷
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摘要：
The single and comparative intradermal tuberculin tests (SITT and CITT) are official in vivo tests for bovine tuberculosis (TB) diagnosis using bovine and avian purified protein derivatives (PPD-B and PPD-A). Infection with bacteria other than Mycobacterium tuberculosis complex (MTC) can result in nonspecific reactions to these tests. We evaluated the performance of the skin test with PPDs and new defined antigens in the guinea pig model. A standard dose (SD) of Rhodococcus equi, Nocardia sp., M. nonchromogenicum, M. monacense, M. intracellulare, M. avium subsp. paratuberculosis, M. avium subsp. avium, M. avium subsp. hominissuis, M. scrofulaceum, M. persicum, M. microti, M. caprae and M. bovis, and a higher dose (HD) of M. nonchromogenicum, M. monacense, M. intracellulare, M. avium subsp. paratuberculosis were tested using PPD-B, PPD-A, P22, ESAT-6-CFP-10-Rv3615c peptide cocktail long (PCL) and fusion protein (FP). The SD of R. equi, Nocardia sp., M. nonchromogenicum, M. monacense, M. intracellulare and M. avium subsp. paratuberculosis did not cause any reactions. The HD of M. nonchromogenicum, M. monacense, M. intracellulare, and M. avium subsp. paratuberculosis and the SD of M. avium subsp. hominissuis, M. scrofulaceum and M. persicum, caused nonspecific reactions (SIT). A CITT interpretation would have considered M. avium complex and M. scrofulaceum groups negative, but not all individuals from M. nonchromogenicum HD, M. monacense HD and M. persicum SD groups. Only animals exposed to M. bovis and M. caprae reacted to PCL and FP. These results support the advantage of complementing or replacing PPD-B to improve specificity without losing sensitivity.
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