Optimal design for the maximization of Penicillium cyclopium lipase production

被引：0

作者：

Vanot G. ^{[1
]}

Deyris V. ^{[1
]}

Guilhem M.-C. ^{[2
]}

Phan Tan Luu R. ^{[2
]}

Comeau L.-C. ^{[1
]}

机构：

[1] Lab. de Chimie Biologique Appliquee, UDESAM, Facult. des Sci. Tech. St-Jerome, 13397 Marseille, Cedex 20, Ave. Escadrille Normandie-Niemen

[2] Laboratoire de la M�thodologie de la Recherche Exp�rimentale, UDESAM, Facult. des Sci. Tech. St-Jerome, 13397 Marseille, Cedex 20, Ave. Escadrille Normandie-Niemen

来源：

Applied Microbiology and Biotechnology | 2001年 / 57卷 / 3期

关键词：

Corn; Lipase; Surface Response; Triacylglycerol; Surface Response Methodology;

D O I：

10.1007/s002530100777

中图分类号：

学科分类号：

摘要：

Penicillium cyclopium triacylglycerol lipase production was maximized in stationary batch culture. We used a surface response methodology based on a Doehlert experimental design to study the effect on the lipase activity released in the culture medium of the three most important factors: substrate concentration, pH and inoculum. Besides reducing the number of experiments required for optimization, this technique allowed us to quantify the lipase activity in any part of the experimental domain. We determined an optimal set of conditions for high lipase production: 1% substrate (corn steep), pH 5.5 and an inoculum of 104 spores/ml. Between conditions giving the minimum and the maximum lipase production, we observed a nine-fold increase of both the predicted and measured values.

引用

页码：342 / 345

页数：3

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