Deep-tissue SWIR imaging using rationally designed small red-shifted near-infrared fluorescent protein

被引:0
|
作者
Olena S. Oliinyk
Chenshuo Ma
Sergei Pletnev
Mikhail Baloban
Carlos Taboada
Huaxin Sheng
Junjie Yao
Vladislav V. Verkhusha
机构
[1] University of Helsinki,Medicum, Faculty of Medicine
[2] Duke University,Department of Biomedical Engineering, School of Engineering
[3] National Institutes of Health,Vaccine Research Center, National Institute of Allergy and Infectious Diseases
[4] Albert Einstein College of Medicine,Department of Genetics and Gruss
[5] Duke University,Lipper Biophotonics Center
来源
Nature Methods | 2023年 / 20卷
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摘要
Applying rational design, we developed 17 kDa cyanobacteriochrome-based near-infrared (NIR-I) fluorescent protein, miRFP718nano. miRFP718nano efficiently binds endogenous biliverdin chromophore and brightly fluoresces in mammalian cells and tissues. miRFP718nano has maximal emission at 718 nm and an emission tail in the short-wave infrared (SWIR) region, allowing deep-penetrating off-peak fluorescence imaging in vivo. The miRFP718nano structure reveals the molecular basis of its red shift. We demonstrate superiority of miRFP718nano-enabled SWIR imaging over NIR-I imaging of microbes in the mouse digestive tract, mammalian cells injected into the mouse mammary gland and NF-kB activity in a mouse model of liver inflammation.
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页码:70 / 74
页数:4
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